Abstract
The purpose of this work was to investigate whether a probiotic bacterium, Lactobacillus plantarum 299v, could affect Escherichia coli-induced passage of mannitol across the intestinal wall. Sprague-Dawley rats were pretreated for one week by either tube feeding with L. plantarum 299v twice daily, free access to L. plantarum 299v by adding the bacterium in the drinking water, or negative control receiving regular feeding. Intestinal segments were mounted in Ussing chambers and the mucosa was exposed to control medium, E. coli, and L. plantarum 299v (alone or together). [14C]Mannitol was added as a marker of intestinal permeability and samples were taken from the serosal side. E. coli exposure induced a 53% increase in mannitol passage across the intestinal wall (P < 0.05). One week of pretreatment with L. plantarum 299v in the drinking water abolished the E. coli-induced increase in permeability. Tube feeding for one week or short-term addition of L. plantarum 299v in the Ussing chambers had no effect on the permeability provoked by E. coli challenge. Notably, L. plantarum 299v itself did not change the intestinal passage of mannitol. These data demonstrate that pretreatment with L. plantarum 299v, which is a probiotic bacterium, protects against E. coli-induced increase in intestinal permeability, and that L. plantarum 299v alone has no influence on the intestinal permeability. Thus, this study supports the concept that probiotics may exert beneficial effects in the gastrointestinal tract.
Similar content being viewed by others
REFERENCES
Berg RD: Bacterial translocation from the gastrointestinal tract. Adv Exp Med Biol 473:11–30, 1999
Pape HC, Dwenger A, Regel G, Auf' m'Kolck M, Gollub F, Wisner D, Sturm JA, Tscherne H: Increased gut permeability after multiple trauma. Br J Surg 81:850–852, 1994
LeVoyer T, Cioffi WGJ, Pratt L: Alterations in intestinal permeability after thermal injury. Arch Surg 127:26–29, 1992
Johnston JD, Harvey CJ, Menzies IS: Gastrointestinal permeability and absorptive capacity in sepsis. Crit Care Med 24:1144–1149, 1996
Olaison G, Sjöberg ÅK, Tagesson C: Abnormal intestinal permeability in Crohn's disease. A possible pathogenic factor. Scand J Gastroenterol 25:321–328, 1990
Nejdfors P, Wang Q, Ekelund M, Weström BR, Jansson O, Jeppsson B: Increased colonic permeability in patients with ulcerative colitis: An in vitro study. Scan J Gastroenterol 33:749–753, 1998
Söderholm JD, Peterson KH, Olaison G, Franzen LE, Weström BR, Magnusson KE, Sjödahl R: Epithelial permeability to proteins in the noninflamed ileum of Crohn's disease? Gastroenterology 117:65–72, 1999
Kops SK, Lowe DK, Bement WM, West AB: Migration of Salmonella typhi through intestinal epithelial monolayers: An in vitro study. Microbiol Immunol 40:799–811, 1996
Spitz J, Yuhan R, Koutsouris A, Blatt C, Alverdy J, Hecht G: Enteropathogenic Escherichia coli adherence to intestinal epithelial monolayers diminshes barrier function. Am J Physiol 268:G374–G379, 1995
Siitonen S, Vapaatalo H, Salminen S, Gordin A, Saxelin M, Wikberg R, Kirkkola AL: Effect of Lactobacillus GG yoghurt in prevention of antibiotic associated diarrhoea. Ann Med 22:57–59, 1990
Majamaa H, Isolauri E, Saxelin M, Vesikari T: Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. J Pediatr Gastroenterol Nutr 20:333–338, 1995
Mao Y, Nobaek S, Kasravi B, Adawi D, Stenram U, Molin G, Jeppsson B: The effects of Lactobacillus strains and oat fiber on methotrexate-induced enterocolitis in rats. Gastroenterology 111:334–344, 1996
Adawi D, Kasravi B, Molin G, Jeppsson B: Effect of Lactobacillus supplementation with and without arginine on liver damage and bacterial translocation in an acute liver injury model in the rat. Hepatology 25:642–647, 1997
Madsen KL, Doyle JS, Jewell LD, Tavernini MM, Fedorak RN: Lactobacillus species prevents colitis in interleukin 10 gene-deficient mice. Gastroenterology 116:1107–1114, 1999
Holzapfel WH, Haberer P, Snel J, Schillinger U, Huis int Veld JJJ: Overview of gut flora and probiotics. Int J Food Microbiol 41:85–101, 1998
Adawi D, Molin G, Ahrné S, Jeppsson B: Modulation of the colonic bacterial flora affects differently bacterial translocation and liver injury in an acute liver injury model. Microb Ecol Health Dis 11:47–54, 1999
Mao Y, Yu J-L, Ljungh Å, Molin G, Jeppsson B: Intestinal immune response to oral administration of Lactobacillus reuteri R2LC, Lactobacillus plantarum DSM 9843, pectin and oatbase on methotrexate-induced enterocolitis in rats. Microb Ecol Health Dis 9:261–270, 1996
Johansson M-L, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S: Administration of different Lactobacillus strains in fermented oatmeal soup: In vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol 5915–20, 1993
Efthymoiu C, Hansen CA: An antigenic analysis of Lactobacillus acidophilus. J Infect Dis 110:258–267, 1962
Ryan CM, Yarmush ML, Burke JF, Tompkins RG: Increased gut permeability early after burns correlates with the extent of burn injury. Crit Care Med 20:1508–1512, 1992
Prescott L M, Harley JP, Klein DA: Microbiology. Dubuque, Iowa, W C Brown Publishers
MacFie J, O'Boyle CJ, Mitchell CJ, Buckley PM, Johnstone D, Sudworth P: Gut origin of sepsis: A prospective study investigating associations between bacterial translocation, gastric microflora and septic morbidity. Gut 45:223–228, 1999
Sedman PC, MacFie J, Sagar P, Mitchell ChJ, May J, Mancey-Jones B, Johnstone D: The prevalence of gut translocation in humans. Gastroenterology 107:643–649, 1994
Krugliak P, Hollander D, Schlaepfer CC, Nguyen H, Ma TY: Mechanisms and sites of mannitol permeability of small and large intestine in the rat. Dig Dis Sci 39:796–801, 1994
Herias MV, Hessle C, Telemo E, Midtvedt T, Hansson LÅ, Wold AE: Immunomodulatory effects of Lactobacillus plantarum colonizing the intestine of gnotobiotic rats. Clin Exp Immunol 116:283–290, 1999
Livesy G, Elia M: Short-chain fatty acids as an energy source in the colon: metabolism and clinical implications. In Physiological and Clinical Aspects of Short-Chain Fatty Acids. JH Cummings, JC Rombeau, T Sakata (eds). Cambridge, Cambridge University Press, 1995, pp 427–481
Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S: Administration of different Lactobacillus strains in fermented oatmeal soup; in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol 59:15–20, 1993
Söderholm JD, Hedman L, Artursson P, Franzén L, Larsson J, Pantzar N, Permert J, Olaison G: Integrity and metabolism of human ileal mucosa in vitro in the Ussing chamber. Acta Physiol Scand 162:47–56, 1998
Armstrong WA: Cellular mechanisms of ion transport in the small intestine. In Physiology of the Gastrointestinal Tract. LR Johnson (ed). New York, Raven Press, 1987, pp 1251–1265
Kurkchubasche AG, Cardona M, Watkins SC, Smith SD, Albanese CT, Simmons RL, Rowe SC, Ford HR: Transmucosal passage of bacteria across rat intestinal epithelium in the Ussing chamber: Effect of nutritional factors and bacterial virulence. Shock 9:121–127, 1998
Albanese CT, Cardona M, Smith SD, Watkins SC, Kurkchubasche AG, Ulman I, Simmons RL, Rowe MI: Role of intestinal mucus in transepithelial passage of bacteria across the intact ileum in vitro. Surgery 116:76–82, 1994
Polentarutti BI, Peterson AL, Sjöberg ÅK, Anderberg EKI, Utter LM, Ungell A-LB: Evaluation of viability of excised rat intestinal segments in the Ussing chamber: Investigation of morphology, electrical parameters and permeability characteristics. Pharmacol Res 16:446–454, 1999
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mangell, P., Nejdfors, P., Wang, M. et al. Lactobacillus plantarum 299v Inhibits Escherichia coli-Induced Intestinal Permeability. Dig Dis Sci 47, 511–516 (2002). https://doi.org/10.1023/A:1017947531536
Issue Date:
DOI: https://doi.org/10.1023/A:1017947531536