Basic–alimentary tractAntibody Blockade of CCL25/CCR9 Ameliorates Early but not Late Chronic Murine Ileitis
Section snippets
Mice
The SAMP1/YitFc substrain was generated after more than 30 generations of continuous inbreeding from 2 breeding pairs of SAMP1/Yit mice provided by Dr S. Matsumoto (Yakult Institute for Microbiological Research, Tokyo, Japan). Mice were kept under specific pathogen-free conditions at the University of Virginia.15 Because most identifiable genes were AKR derived, age-matched AKR/J mice were used as controls.16 Fecal samples from SAMP1/YitFc mice were consistently negative for Helicobacter
CCR9 Expression Is Increased in SAMP1/YitFc Mice During Disease Induction
We examined the surface expression of CCR9 on CD4+ and CD8+ cells isolated from the spleen and MLN of SAMP1/YitFc mice and compared it with that of noninflamed AKR control mice at 4–6 weeks of age. Phycoerythrin-labeled isotype antibody (mean fluorescence index [MFI] < 101, not shown) and lymphocytes isolated from the respective organs of CCR9-deficient mice were used as controls (Figure 1, dashed lines). CCR9+ T cells were identified in MLN and spleen of both control AKR and SAMP1YitFc mice (
Discussion
We investigated whether CCL25/CCR9 play a role in chronic inflammatory trafficking to the small intestine using the SAMP1/YitFc model of chronic ileitis.15, 26 This strain differs from other animal models of inflammatory bowel disease, because the site of inflammation coincides with the restricted location of CCL25 expression.31 We showed that the levels of CCL25 increased in inflamed small intestine compared with noninflamed mice and that further increases occur as the inflammation progresses.
References (45)
IBD: etiology and pathogenesis
Gastroenterology
(1998)- et al.
TECK: a novel CC chemokine specifically expressed by thymic dendritic cells and potentially involved in T cell development
Immunity
(1997) - et al.
Chemokines and the tissue-specific migration of lymphocytes
Immunity
(2002) - et al.
CCR9-positive lymphocytes and thymus-expressed chemokine distinguish small bowel from colonic Crohn’s disease
Gastroenterology
(2001) - et al.
Identification of a quantitative trait locus for ileitis in a spontaneous mouse model of Crohn’s disease: SAMP1/YitFc
Gastroenterology
(2003) - et al.
Antibody blockade of ICAM-1 and VCAM-1 ameliorates inflammation in the SAMP-1/Yit adoptive transfer model of Crohn’s disease in mice
Gastroenterology
(2001) - et al.
Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization
J Biol Chem
(2001) - et al.
Differential effects of CC chemokines on CC chemokine receptor 5 (CCR5) phosphorylation and identification of phosphorylation sites on the CCR5 carboxyl terminus
J Biol Chem
(1999) - et al.
Emergence of perianal fistulizing disease in the SAMP1/YitFc mouse, a spontaneous model of chronic ileitis
Gastroenterology
(2003) - et al.
Proinflammatory effects of TH2 cytokines in a murine model of chronic small intestinal inflammation
Gastroenterology
(2005)
Lymphocyte trafficking and regional immunity
Adv Immunol
MAdCAM-1 expressed in chronic inflammatory liver disease supports mucosal lymphocyte adhesion to hepatic endothelium (MAdCAM-1 in chronic inflammatory liver disease)
Hepatology
Aberrant binding of lamina propria lymphocytes to vascular endothelium in inflammatory bowel diseases
Gastroenterology
The chemokine system: redundancy for robust outputs
Immunol Today
Regulated production of interferon-inducible T-cell chemoattractants by human intestinal epithelial cells
Gastroenterology
Chemokines regulate lymphocyte homing to the intestinal mucosa
Gastroenterology
Safety and efficacy of recombinant human interleukin 10 in chronic active Crohn’s diseaseCrohn’s Disease IL-10 Cooperative Study Group
Gastroenterology
Inflammatory bowel disease (1)
N Engl J Med
Chemokines in lymphocyte trafficking and intestinal immunity
Microcirculation
T-cell function and migrationTwo sides of the same coin
N Engl J Med
The intestinal chemokine thymus-expressed chemokine (CCL25) attracts IgA antibody-secreting cells
J Exp Med
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells
J Immunol
Cited by (126)
Leukocyte trafficking to the intestinal barrier in health and disease
2022, Cell Movement in Health and DiseaseRole of CC chemokine receptor 9 in the progression of murine and human non-alcoholic steatohepatitis
2021, Journal of HepatologyTherapeutic and Diagnostic Implications of T Cell Scarring in Celiac Disease and Beyond
2019, Trends in Molecular Medicine
Supported by US Public Health Service/National Institutes of Health grants DK067254 and DK057880, by a Harold Amos MMFDP grant from the Robert Wood Johnson Foundation (to J.R-N.), by Deutsche Forschungsgemeinschaft grants SFB402/B7 (to M.O.) and GRK335 to (N.I.), and by the Administrative, Morphology, and Immunology Cores of the University of Virginia Digestive Health Research Center (DK 56703).