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Relation of antigenic structure of cereal proteins to their toxicity in coeliac patients

Published online by Cambridge University Press:  07 March 2008

P. J. Ciclitira
Affiliation:
Gastroenterology Unit, Department of Medicine, Guy's Hospital, United Medical School of Guy's and St Thomas' Hospitals, London SE1 9RT
H. J. Ellis
Affiliation:
Gastroenterology Unit, Department of Medicine, Guy's Hospital, United Medical School of Guy's and St Thomas' Hospitals, London SE1 9RT
D. J. Evans
Affiliation:
Department of Histopathology, Hammersmith Hospital, LondonW12 0HS
E. S. Lennox
Affiliation:
MRC Laboratory of Molecular Biology, CambridgeCB2 2QH
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Abstract

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1. Unfractionated gliadin and its α-, β-, γ- and ω-gliadin subfractions were used as rabbit immunogens. The antisera were characterized by (1) Ouchterlony double diffusion, (2) binding of 125I-labelled gliadin subfractions, (3) inhibition by several gliadin subfractions of binding between γgliadin antiserum and 125I-labeIled γgliadin.

2. Double diffusion showed identical cross-reactivity between the antisera and the gliadin subfractions with the exception of ω-gliadin. Precipitin lines of partial identity with gliadin were observed against rye secalins and barley hordeins but not oat avenins or maize zeins.

3. Binding was observed between unfractionated 125I-labelledα-, β-, γ- and ω-gliadins and all the antisera. There was binding of 125I-labelled ω-gliadin to the ω-gliadin antiserum but poor binding of 125I-labelled ω-gliadin to unfractionated α-, β- and γ-gliadin antisera. Competitive inhibition of binding between 125I-labelled γ-gliadin and γ-gliadin antiserum diluted 1:250 (v/v) demonstrated similar competition between α-, β- and γ-gliadins and this antiserum but poor competition between ω-gliadin, wheat glutenins, albumins and globulins, rye secalins, barley hordeins and oat avenins.

4. These findings suggest that there is a good correlation between the antigenic structure of gliadin proteins and their toxicity to patients with coeliac disease.

Type
Papers of direct relevance to Clinical and Human Nutrition
Copyright
Copyright © The Nutrition Society 1985

References

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