Abstract
The aim of the present study was to investigate the role of anti-inflammation for MSCs transplantation in rat models of myocardial infarction. Rats with AMI induced by occlusion of the left coronary artery were randomized to MSCs transplantation group, MI group and sham operated group. The effects of MSCs transplantation on cardiac inflammation and left ventricular remodeling in non-infarcted zone were observed after 4 weeks of MI. We found that MSC transplantation (1) decreased protein production and gene expression of inflammation cytokines TNF-alpha, IL-1beta and IL-6, (2) inhibited deposition of type I and III collagen, as well as gene and protein expression of MMP-1 and TIMP-1, (3) attenuated LV cavitary dilation and transmural infarct thinning, thus prevent myocardial remodeling after myocardial infarction, and (4) increased EF, FS, LVESP and dp/dtmax (P < 0.01), decreased LVDd, LVEDV, LVEDP (P < 0.05). Anti-inflammation role for MSCs transplantation might partly account for the cardiac protective effect in ischemic heart disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Collagen Type I / metabolism
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Collagen Type III / metabolism
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Echocardiography
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Gene Expression / immunology
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Hypertrophy, Left Ventricular / diagnostic imaging
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Hypertrophy, Left Ventricular / immunology
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Hypertrophy, Left Ventricular / therapy
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Interleukin-1beta / genetics
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Interleukin-6 / genetics
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Male
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Matrix Metalloproteinase 1 / genetics
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Matrix Metalloproteinase 1 / metabolism
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Mesenchymal Stem Cell Transplantation*
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Mesenchymal Stem Cells / immunology*
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Myocardial Infarction / diagnostic imaging
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Myocardial Infarction / immunology*
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Myocardial Infarction / therapy*
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Myocarditis / diagnostic imaging
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Myocarditis / immunology
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Myocarditis / therapy
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Myocardium / metabolism
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Myocardium / pathology
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Rats
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Rats, Sprague-Dawley
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Tissue Inhibitor of Metalloproteinase-1 / genetics
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Tissue Inhibitor of Metalloproteinase-1 / metabolism
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Tumor Necrosis Factor-alpha / genetics
Substances
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Collagen Type I
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Collagen Type III
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Interleukin-1beta
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Interleukin-6
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Tissue Inhibitor of Metalloproteinase-1
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Tumor Necrosis Factor-alpha
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Matrix Metalloproteinase 1