Abstract
Trefoil peptides, a growing family of secretory molecules, have been identified mainly in the gastrointestinal tract of humans, rodents and amphibians. In the present study, the nucleotide sequence of a large portion (81%) of the gene encoding murine intestinal trefoil factor (mITF) and its whole genomic organization were determined. The mITF gene contains three exons distributed over 5 kb of genomic DNA. The genomic sequence is highly conserved, as compared with that of the rat and human ITF, and contains several AP-1-binding sites, the consensus binding site for the transcription factor Sp1, and a sequence homologous to a heat-shock element. Fluorescence in situ hybridization was used to assign ITF to chromosome 17 of the murine genome, a region syntenic with the trefoil gene cluster on human chromosome 21q22.3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Chromosome Mapping
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Cloning, Molecular
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Conserved Sequence
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Growth Substances / chemistry
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Growth Substances / genetics*
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Growth Substances / metabolism
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Heat-Shock Proteins / chemistry
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Heat-Shock Proteins / genetics
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Intestinal Mucosa / metabolism
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Mice / genetics*
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Molecular Sequence Data
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Mucins*
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Muscle Proteins*
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Neuropeptides*
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Peptides / chemistry
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Peptides / genetics*
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Peptides / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Transcription Factor AP-1 / metabolism
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Transcription Factors / metabolism
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Trefoil Factor-2
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Trefoil Factor-3
Substances
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Growth Substances
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Heat-Shock Proteins
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Mucins
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Muscle Proteins
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Neuropeptides
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Peptides
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TFF3 protein, rat
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Transcription Factor AP-1
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Transcription Factors
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Trefoil Factor-2
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Trefoil Factor-3