Direct secretory effect of interleukin-1 via type I receptors in human colonic mucous epithelial cells (HT29-C1.16E)

Gut. 1996 Feb;38(2):240-2. doi: 10.1136/gut.38.2.240.

Abstract

The stable differentiated human colonic epithelial cell line, HT29-C1.16E, was used to study the effects of interleukin-1 (IL-1) on mucin exocytosis. The main findings include: (a) IL-1 stimulated a rapid release of mucin from filter grown HT29-C1.16E cells, this effect being dose related; (b) this secretory effect was abolished in the presence of the blocking monoclonal antibody M4 specific for IL-1 receptors type I, showing that IL-1 receptors type I mediated IL-1 action; (c) experiments based on chamber cultures showed that these receptors were located on the basolateral membranes of HT29-C1.16E cells; (d) finally, mRNA for IL-1 receptors type I were detected by reverse transcriptase-polymerase chain reaction in these cells. To extend these findings to the in vivo situation, the rapid stimulatory effect of IL-1 on mucin exocytosis may contribute to the wash out of noxious agents during mucosal inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / drug effects
  • Cell Line
  • Colon / chemistry*
  • Colon / cytology
  • Exocytosis / drug effects
  • HT29 Cells
  • Humans
  • Interleukin-1 / pharmacology*
  • Mucins / drug effects*
  • Mucins / metabolism
  • Receptors, Interleukin-1 / analysis
  • Receptors, Interleukin-1 / drug effects*

Substances

  • Antibodies, Monoclonal
  • Interleukin-1
  • Mucins
  • Receptors, Interleukin-1