PT  - JOURNAL ARTICLE
AU  - Melanie Uhde
AU  - Mary Ajamian
AU  - Giacomo Caio
AU  - Roberto De Giorgio
AU  - Alyssa Indart
AU  - Peter H Green
AU  - Elizabeth C Verna
AU  - Umberto Volta
AU  - Armin Alaedini
TI  - Intestinal cell damage and systemic immune activation in individuals reporting sensitivity to wheat in the absence of coeliac disease
AID  - 10.1136/gutjnl-2016-311964
DP  - 2016 Jul 21
TA  - Gut
PG  - gutjnl-2016-311964
4099  - http://gut.bmj.com/content/early/2016/07/21/gutjnl-2016-311964.short
4100  - http://gut.bmj.com/content/early/2016/07/21/gutjnl-2016-311964.full
AB  - Objective Wheat gluten and related proteins can trigger an autoimmune enteropathy, known as coeliac disease, in people with genetic susceptibility. However, some individuals experience a range of symptoms in response to wheat ingestion, without the characteristic serological or histological evidence of coeliac disease. The aetiology and mechanism of these symptoms are unknown, and no biomarkers have been identified. We aimed to determine if sensitivity to wheat in the absence of coeliac disease is associated with systemic immune activation that may be linked to an enteropathy.Design Study participants included individuals who reported symptoms in response to wheat intake and in whom coeliac disease and wheat allergy were ruled out, patients with coeliac disease and healthy controls. Sera were analysed for markers of intestinal cell damage and systemic immune response to microbial components.Results Individuals with wheat sensitivity had significantly increased serum levels of soluble CD14 and lipopolysaccharide (LPS)-binding protein, as well as antibody reactivity to bacterial LPS and flagellin. Circulating levels of fatty acid-binding protein 2 (FABP2), a marker of intestinal epithelial cell damage, were significantly elevated in the affected individuals and correlated with the immune responses to microbial products. There was a significant change towards normalisation of the levels of FABP2 and immune activation markers in a subgroup of individuals with wheat sensitivity who observed a diet excluding wheat and related cereals.Conclusions These findings reveal a state of systemic immune activation in conjunction with a compromised intestinal epithelium affecting a subset of individuals who experience sensitivity to wheat in the absence of coeliac disease.