RT Journal Article
SR Electronic
T1 Fungal microbiota dysbiosis in IBD
JF Gut
JO Gut
FD BMJ Publishing Group Ltd and British Society of Gastroenterology
SP 1039
OP 1048
DO 10.1136/gutjnl-2015-310746
VO 66
IS 6
A1 Harry Sokol
A1 Valentin Leducq
A1 Hugues Aschard
A1 Hang-Phuong Pham
A1 Sarah Jegou
A1 Cecilia Landman
A1 David Cohen
A1 Giuseppina Liguori
A1 Anne Bourrier
A1 Isabelle Nion-Larmurier
A1 Jacques Cosnes
A1 Philippe Seksik
A1 Philippe Langella
A1 David Skurnik
A1 Mathias L Richard
A1 Laurent Beaugerie
YR 2017
UL http://gut.bmj.com/content/66/6/1039.abstract
AB Objective The bacterial intestinal microbiota plays major roles in human physiology and IBDs. Although some data suggest a role of the fungal microbiota in IBD pathogenesis, the available data are scarce. The aim of our study was to characterise the faecal fungal microbiota in patients with IBD.Design Bacterial and fungal composition of the faecal microbiota of 235 patients with IBD and 38 healthy subjects (HS) was determined using 16S and ITS2 sequencing, respectively. The obtained sequences were analysed using the Qiime pipeline to assess composition and diversity. Bacterial and fungal taxa associated with clinical parameters were identified using multivariate association with linear models. Correlation between bacterial and fungal microbiota was investigated using Spearman's test and distance correlation.Results We observed that fungal microbiota is skewed in IBD, with an increased Basidiomycota/Ascomycota ratio, a decreased proportion of Saccharomyces cerevisiae and an increased proportion of Candida albicans compared with HS. We also identified disease-specific alterations in diversity, indicating that a Crohn's disease-specific gut environment may favour fungi at the expense of bacteria. The concomitant analysis of bacterial and fungal microbiota showed a dense and homogenous correlation network in HS but a dramatically unbalanced network in IBD, suggesting the existence of disease-specific inter-kingdom alterations.Conclusions Besides bacterial dysbiosis, our study identifies a distinct fungal microbiota dysbiosis in IBD characterised by alterations in biodiversity and composition. Moreover, we unravel here disease-specific inter-kingdom network alterations in IBD, suggesting that, beyond bacteria, fungi might also play a role in IBD pathogenesis.