Table 1

Specifications of the primer sets used to analyse cytokine mRNA expression

Cytokine Primer set Annealing temperature PCR product size (bp)
β-actin5′-TGACGGGGTCACCCACACTGTGCCCATCTA-3′72661
5′-CTAGAAGCATTGCGGTGGACGATGGAGGG-3′
IL-1α5′-GTCTCTGAATCAGAAATCCTTCTATC-3′60421
5′-CATGTCAAATTTCACTGCTTCATCC-3′
IL-1β5′-AAACAGATGAAGTGCTCCTTCCAGG-3′60391
5′-TGGAGAACACCACTTGTTGCTCCA-3′
IL-25′-GAATGGAATTAATAATTACAAGAATCCC-3′60222
5′-TGTTTCAGATCCCTTTAGTTCCAG-3′
IL-35′-TCCAAACATGAGCCGCCTGCC-3′60211
5′-CATCAGAATGTCTTGGTCTTC-3′
IL-45′-AACACAACTGAGAAGGAAACCTTC-3′65276
5′-GCTCGAACACTTTGAATATTTCTC-3′
IL-55′-GCTTCTGCATTTGAGTTTGCTAGCT-3′65293
5′-TGGCCGTCAATGTATTTCTTTATTAAG-3′
IL-65′-ATGAACTCCTTCTCCACAAGCGC-3′60628
5′-GAAGAGCCCTCAGGCTGGACTG-3′
IL-75′-ATGTTCCATGTTTCTTTTAGGTATATCT-3′60680
5′-TGCATTTCTCAAATGCCCTAATCCG-3′
IL-85′-ATGACTTCCAAGCTGGCCGTGGCT-3′60289
5′-TCTCAGCCCTCTTCAAAAACTTCTC-3′
IL-95′-TCACCATGGTTCTGGCCATGGTCCTTACCTC-3′60487
5′-ATAATATTTCATCTTCATATCTTGCCTCTCA-3′
IL-105′-ATGCCCCAAGCTGAGAACCAAGACCCA-3′72352
5′-TCTCAAGGGGCTGGGTCAGCTATCCCA-3′
IL-115′-ACTGCTGCTGCTGAAGACTCGGCTGTGA-3′62322
5′-ATGGGGAAGAGCCAGGGCAGAAGTCTGT-3′
IL-12p405′-AGTGTCAAAAGCAGCAGAGG-3′60363
5′-AACGCAGAATGTCAGGGAG-3′
IL-135′-GCATGGTATGGAGCATCAAC-3′60371
5′-ATTTTACCCCTCCCTAACCCT-3′
IL-145′-CCCTCCAGTTCTTTATCC-3′55224
5′-CAACAGCTTGCCCTATAC-3′
IL-155′-TGGAGCACAGAAATCAATG-3′55265
5′-GAAACAGCCCAAAATGAAG-3′
IFN-γ5′-ATGAAATATACAAGTTATATCTTGGCTTT-3′60501
5′-GATGCTCTTCGACCTCGAAACAGCAT-3′
TNF-α5′-CGGGACGTGGAGCTGGCCGAGGAG-3′72355
5′-CACCAGCTGGTTATCTCTCAGCTC-3′
TGF-β15′-GCCCTGGACACCAACTATTGCT-3′65161
5′-AGGCTCCAAATGTAGGGGCAGG-3′
GM-CSF5′-GAGCATGTGAATGCCATCCAGGAG-3′55390
5′-CTCCTGGACTGGCTCCCAGCAGTCAAA-3′