Cytokine
|
Primer set
|
Annealing temperature
|
PCR product size (bp)
|
β-actin | 5′-TGACGGGGTCACCCACACTGTGCCCATCTA-3′ | 72 | 661 |
| 5′-CTAGAAGCATTGCGGTGGACGATGGAGGG-3′ | | |
IL-1α | 5′-GTCTCTGAATCAGAAATCCTTCTATC-3′ | 60 | 421 |
| 5′-CATGTCAAATTTCACTGCTTCATCC-3′ | | |
IL-1β | 5′-AAACAGATGAAGTGCTCCTTCCAGG-3′ | 60 | 391 |
| 5′-TGGAGAACACCACTTGTTGCTCCA-3′ | | |
IL-2 | 5′-GAATGGAATTAATAATTACAAGAATCCC-3′ | 60 | 222 |
| 5′-TGTTTCAGATCCCTTTAGTTCCAG-3′ | | |
IL-3 | 5′-TCCAAACATGAGCCGCCTGCC-3′ | 60 | 211 |
| 5′-CATCAGAATGTCTTGGTCTTC-3′ | | |
IL-4 | 5′-AACACAACTGAGAAGGAAACCTTC-3′ | 65 | 276 |
| 5′-GCTCGAACACTTTGAATATTTCTC-3′ | | |
IL-5 | 5′-GCTTCTGCATTTGAGTTTGCTAGCT-3′ | 65 | 293 |
| 5′-TGGCCGTCAATGTATTTCTTTATTAAG-3′ | | |
IL-6 | 5′-ATGAACTCCTTCTCCACAAGCGC-3′ | 60 | 628 |
| 5′-GAAGAGCCCTCAGGCTGGACTG-3′ | | |
IL-7 | 5′-ATGTTCCATGTTTCTTTTAGGTATATCT-3′ | 60 | 680 |
| 5′-TGCATTTCTCAAATGCCCTAATCCG-3′ | | |
IL-8 | 5′-ATGACTTCCAAGCTGGCCGTGGCT-3′ | 60 | 289 |
| 5′-TCTCAGCCCTCTTCAAAAACTTCTC-3′ | | |
IL-9 | 5′-TCACCATGGTTCTGGCCATGGTCCTTACCTC-3′ | 60 | 487 |
| 5′-ATAATATTTCATCTTCATATCTTGCCTCTCA-3′ | | |
IL-10 | 5′-ATGCCCCAAGCTGAGAACCAAGACCCA-3′ | 72 | 352 |
| 5′-TCTCAAGGGGCTGGGTCAGCTATCCCA-3′ | | |
IL-11 | 5′-ACTGCTGCTGCTGAAGACTCGGCTGTGA-3′ | 62 | 322 |
| 5′-ATGGGGAAGAGCCAGGGCAGAAGTCTGT-3′ | | |
IL-12p40 | 5′-AGTGTCAAAAGCAGCAGAGG-3′ | 60 | 363 |
| 5′-AACGCAGAATGTCAGGGAG-3′ | | |
IL-13 | 5′-GCATGGTATGGAGCATCAAC-3′ | 60 | 371 |
| 5′-ATTTTACCCCTCCCTAACCCT-3′ | | |
IL-14 | 5′-CCCTCCAGTTCTTTATCC-3′ | 55 | 224 |
| 5′-CAACAGCTTGCCCTATAC-3′ | | |
IL-15 | 5′-TGGAGCACAGAAATCAATG-3′ | 55 | 265 |
| 5′-GAAACAGCCCAAAATGAAG-3′ | | |
IFN-γ | 5′-ATGAAATATACAAGTTATATCTTGGCTTT-3′ | 60 | 501 |
| 5′-GATGCTCTTCGACCTCGAAACAGCAT-3′ | | |
TNF-α | 5′-CGGGACGTGGAGCTGGCCGAGGAG-3′ | 72 | 355 |
| 5′-CACCAGCTGGTTATCTCTCAGCTC-3′ | | |
TGF-β1 | 5′-GCCCTGGACACCAACTATTGCT-3′ | 65 | 161 |
| 5′-AGGCTCCAAATGTAGGGGCAGG-3′ | | |
GM-CSF | 5′-GAGCATGTGAATGCCATCCAGGAG-3′ | 55 | 390 |
| 5′-CTCCTGGACTGGCTCCCAGCAGTCAAA-3′ | | |