Table 3

Measurement of platelet activating factor (PAF) content in the ileal wall by thin layer chromatography (TLC) and radioimmunoassay (RIA) in saline and trinitrobenzenesulphonic acid (TNB) treated puinea pigs

		Ileal PAF content after TNB treatment (ng/mg tissue)
Day of inflammation	Ileal PAF content after saline treatment (ng/mg tissue; TLC)	TLC	RIA
Controls (day 0)	109 (13)	109 (13)	123 (17)
1	195 (19)^*	165 (22)	187 (11)
2	163 (3)	237 (6)^** ^†	240 (22)^*
3	261 (32)^***	348 (31)^*** ^†	300 (10)^**
4	174 (18)^*	295 (28)^*** ^††	317 (24)^**
5	130 (27)	337 (49)^*** ^†† ^†	322 (43)^**
6	132 (26)	290 (32)^** ^†† ^†	267 (67)^*
10	113 (9)	200 (9)^** ^†	192 (35)
15	109 (16)	215 (30)^* ^†	237 (16)^*

Values are mean (SEM) (triplicate experiments on samples from five different animals).
Values significantly different from those measured as day 0: ^*p<0.05,^**p<0.01, ^***p<0.001.
Values significantly different from those measured on the same day, in samples from saline treated animals (applies only to TLC measurements):^†p<0.05, ^††p<0.01, ^†† ^†p<0.001.