Sequence of primers | Size of RT-PCR products (bp) | References | Enzymes used for confirmation | Expected size of cleavage products (bp) | |
LBP | 5′-TGCAACAACAAGGGAGCTCAC-3′ | 450 bp | 16 | PstI | 348+102 |
5′-TCCATCAACCATTTTTCCAT-3′ | |||||
Integrins | |||||
α1 | 5′-GCCCCAGTAGAGGACATGTTT-3′ | 324 bp | 13 | HinfI | 182+142 |
5′-ACATATTCCAGTTGTATAATG-3′ | |||||
α6 | 5′-TCCCTGAACCTAACGGAGTCT-3′ | 254 bp | 14 | XhoI | 196+58 |
5′-ATGTCCAAGTAGTTCAGTTTG-3′ | |||||
α6A/B | 5′-GAACTGTGTGAACATCAGA-3′ | α6A 500 bp | 14, 18 | ||
5′-ATCCTTACAGCATGGTATCG-3′ | α6B 370 bp | ||||
β1 | 5′-ACAGCAGAGAAGCTGAAGCCA-3 | 306 bp | 15 | BglII | 198+108 |
5′-GAGCTTAGCTGGTGTTGTGC-3′ | |||||
β1A | 5′-AGAATCCAGAGTGTCCCACTGG-3′ | 238 bp | 15, 19 | ||
5′-TTCCCTCATACTTCGGATTGA-3′ | |||||
β1B | 5′-ACTTATGTATTAGCTGTCAG-3′ | 272 bp | 15, 19 | ||
5′-CATTGAATAGCTTGCTACCA-3′ | |||||
β-actin | 5′-GTGGGGCGCCCCAGGCACCA-3′ | 540 bp | 17 | TaqI | 430+110 |
5′-CTCCTTAATTGTCACGCACGATTC-3′ |
RT-PCR, reverse transcription polymerase chain reaction; LBP, laminin binding protein.