Table 1

Oligonucleotide primers used for RT-PCR analysis

Sequence of primersSize of RT-PCR products (bp)ReferencesEnzymes used for confirmationExpected size of cleavage products (bp)
LBP5′-TGCAACAACAAGGGAGCTCAC-3′450 bp16PstI348+102
5′-TCCATCAACCATTTTTCCAT-3′
Integrins
α15′-GCCCCAGTAGAGGACATGTTT-3′324 bp13HinfI182+142
5′-ACATATTCCAGTTGTATAATG-3′
 α65′-TCCCTGAACCTAACGGAGTCT-3′254 bp14XhoI196+58
5′-ATGTCCAAGTAGTTCAGTTTG-3′
 α6A/B5′-GAACTGTGTGAACATCAGA-3′α6A 500 bp14, 18
5′-ATCCTTACAGCATGGTATCG-3′α6B 370 bp
 β15′-ACAGCAGAGAAGCTGAAGCCA-3306 bp15BglII198+108
5′-GAGCTTAGCTGGTGTTGTGC-3′
 β1A5′-AGAATCCAGAGTGTCCCACTGG-3′238 bp15, 19
5′-TTCCCTCATACTTCGGATTGA-3′
 β1B5′-ACTTATGTATTAGCTGTCAG-3′272 bp15, 19
5′-CATTGAATAGCTTGCTACCA-3′
β-actin5′-GTGGGGCGCCCCAGGCACCA-3′540 bp17TaqI430+110
5′-CTCCTTAATTGTCACGCACGATTC-3′
  • RT-PCR, reverse transcription polymerase chain reaction; LBP, laminin binding protein.