Syngeneic grafts1-152 | Rejected grafts (59%, 44–74)1-152 | Tolerated grafts (99%, 89–99)1-152 | |
TCRαβ+CD25+ | 22% (20–31) | 22.2% (14–33) | 15.5% (8–24) |
(n=3) | (n=7) | (n=3) | |
CD8αβ+CD25+ | 2.5% (2–2.5) | 21.3% (9–26)1-150 | 1.1% (0–2)1-150 |
(n=3) | (n=3) | (n=3) | |
CD4+CD25+ | 19% (16–28) | 18.3% (11–28) | 10.2% (7–20) |
(n=3) | (n=6) | (n=3) | |
NKR-P1+CD25+ | 0.8% (0–5) | 5.1% (2–6) | 0.18% (0–0.4) |
(n=3) | (n=3) | (n=3) | |
PVG+CD25+ | 30% (24–36) | 29.6% (19–42) | 11.4% (10–24)1-150 |
(n=3) | (n=7) | (n=5) |
↵1-151 Data are percentage of positive cells among graft infiltrating cells of recipient origin (median, range). n = number of animals analysed.
↵1-152 The percentage of cells of recipient origin (median, range) were determined in rejected and tolerated grafts using the OX-27 biotinylated monoclonal antibody as indicated in methods. In syngeneic grafts cells of donor and recipient origin could not be distinguished and immunofluorescence was performed on total cells.
↵1-150 p<0.05 (Mann-Whitney U test).