Phytohaemagglutinin (PHA) stimulated interferon γ (IFN-γ) release in lamina propria T lymphocytes (LPLs) and peripheral blood lymphocytes (PBLs) after 24 hours of culture
| IFN-γ secretion (pg/ml) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Medium alone | Medium + anti-IL-2 | Medium + CsA | ||||||
| Stimulus | T-LPLs | T-PBLs | T-LPLs | T-PBLs | T-LPLs | T-PBLs | ||
| Medium + anti-CD2 | 500 (40) | 2500 (84) | 120 (40) | 1600 (65.5) | 50 (10) | 42 (10) | ||
| Medium + anti-CD28 | 30 (5.5) | 34 (2.2) | ND | 25 (2.8) | ND | ND | ||
| IL-12 (100 pg/ml) | 65 (6.0) | 74 (6.0) | 68.5 (3.8) | 79 (3.5) | 64.6 (4.2) | 72 (6.6) | ||
Results are expressed as mean (SD) from five representative experiments.
PHA stimulated lymphocytes were cultured with or without the initial addition of interleukin (IL)-12 (100 pg/ml) or anti-CD2 (1:1000 final dilution) or anti-CD28 (at a final concentration 1μg/ml) antibody, in the presence or absence of cyclosporin A (CsA) (0.1 μg/ml final concentration) or anti-IL-2 antibody (1:100 final dilution).
ND, not detectable.