Effect of hyperbaric oxygen (HBO) on colonic weight, eicosanoid generation, and myeloperoxidase (MPO) and nitric oxide synthase (NOS) activities
| HBO | |||
| Treatment | Control | 1 day | 7 days |
| No of rats | 12–22 | 8–10 | 6 |
| Weight (g/10 cm) | 0.61 (0.01) | 0.58 (0.02) | 0.60 (0.03) |
| MPO (U/g) | 0.98 (0.09) | 0.76 (0.13) | 1.60 (0.20) |
| NOS (nmol/g/min) + Ca2+ | 2.93 (0.17) | 3.30 (0.18) | 1.10 (0.07)* |
| NOS (nmol/g/min) − Ca2+ | 2.06 (0.10) | 2.90 (0.10) | 0.32 (0.09)* |
| PGE2(ng/g) | 39.00 (1.76) | 26.00 (1.48)* | 33.70 (4.30) |
| LTB4 (ng/g) | 1.00 (0.10) | NT | 1.8 (0.25) |
Results are expressed as mean (SEM).
Rats were treated with HBO as described in Materials and Methods. Rats were killed after one or seven days as indicated. The distal 10 cm long colonic segment was isolated and the mucosa was scraped for determination of eicosanoid generation, and MPO and NOS activities.
Significantly different from control, untreated rats, *p<0.05.
PGE2, prostaglandin E2; LTB4, leukotriene B4; NT, not tested.