Table 1

Oligonucleotide primers used in this study

Oligonucleotide Gene Strand Sequence (5′→3′) Strain of origin
HP1 glmM +GGATAAGCTTTTAGGGGTGTTAGGGG85P
HP2 glmM −GCTTACTTTCTAACACTAACGCGC85P
CAG11-150 cagA +GATAACAGGCAAGCTTTTGAGGGACCUG 17874
CAG21-150 cagA −CCATGAATTTTTGATCCGTTCGGCCUG 17874
CAG31-150 cagE +GTTACATCAAAAATAAAAGGAAGCGCCUG 17874
CAG41-150 cagE −CAATAATTTTGAAGAGTTTCAAAGGCCUG 17874
CAG51-150 cagM +ACAAATACAAAAAAGAAAAAGAGGCCCUG 17874
CAG61-150 cagM −ATTTTTCAACAAGTTAGAAAAAGCCCCUG 17874
CAG7 cagQ −TTTGGTTGGTAATGGTTTTGGTAGCCCUG 17874
CAG81-150 tnpB +CGCTCTCCCTAAATTCAAAGAGGGCCCUG 17874
CAG91-150 tnpB −AGCTAGGGAAAAATCTGTCTATGCCCCUG 17874
CAG101-150 tnpA +ATCAGTCCAAAAAGTTTTTTCTTTCCCCUG 17874
CAG111-150 tnpA −TAAGGGGGTATATTTCAACCAACCGCCUG 17874
CAG12 cagS +GCAATCATTGAGAAGAGTTTTCGCNCTC 11638
CAG131-150 cagT +TCTAAAAAGATTACGCTCATAGGCGNCTC 11638
CAG141-150 cagT −CTTTGGCTTGCATGTTCAAGTTGCCNCTC 11638
CAG151-150 ORF13+CGTTCATGTTCCATACATCTTTGGCNCTC 11638
CAG161-150 ORF13−GATTTATAGCGATCTAAGAAACCGCNCTC 11638
CAG171-150 ORF10+AATAGTGCTTTCTTTAGGATTAGCGNCTC 11638
CAG181-150 ORF10−CCGATTTAATCCTTTCGCTTATGTGNCTC 11638
CAG19ORF6+GTTTTATATTTTTTGCTATAATGGCNCTC 11638
CAG20ORF6−AGTCTCTTTAGCTTCAGTTGGTTCGNCTC 11638
CAG211-150 ORF6+TTCAAAAACAATTTGACTGATAAGCG26695
CAG221-150 ORF6−TTGGTTCGTTGGTAACGCTTGTTGC26695

  • ↵1-150 Oligonucleotides used to generate probes for colony hybridisation.