Oligonucleotide primers used in this study
| Primer designation | Primer sequence | Location (reference) |
|---|---|---|
| cagA-F | 5′-AGCTGCAGCATGACTAACGAAAACTATTGACCAAC-3′ | (1) |
| cagA-R | 5′-AGCTGCAGCTTAAGATTTTTGGAAACCACCTTTT-3′ | (1) |
| cagB-F | 5′-ATGGAAAACAAATCAATAGG-3′ | (18495–185141-150) |
| cagB-R | 5′-CTACTTGTCCCAACCATTTT-3′ | (18287–182681-150) |
| cagC-F | 5′-ATGAAATTTTTTACAAGAAT-3′ | (18142–181231-150) |
| cagC-R | 5′-TTAGCTAGCTCCTCCACCCT-3′ | (17795–178141-150) |
| cagE-F | 5′-TCTATAAAGAGAGGGGTGTT-3′ | (17170–171511-150) |
| cagE-R | 5′-GGCTAATCTTTGGTAATCAG-3′ | (14451–144691-150) |
| cagM-F | 5′-ATGCTTGCAAAAATTGTTTT-3′ | (7772–77911-150) |
| cagM-R | 5′-CTATTCAAAGGGATTATTCT-3′ | (8902–88831-150) |
| cagN-F | 5′-GTCGCTCTTTATTCTTTAGT-3′ | (9837–98181-150) |
| cagN-R | 5′-CTATTTTTTTCCATGAGCGA-3′ | (8947–89661-150) |
| cagQ-F | 5′-CGTGTTTTAGTAGGAAGCAT-3′ | (6570–65511-150) |
| cagQ-R | 5′-CTACAGTCTTACTTGAGAGA-3′ | (6190–62091-150) |
| cagT-F | 5′-ATGAAAGTGAGAGCAAGTGT-3′ | (344–3631-150) |
| cagT-R | 5′-TCACTTACCACTGAGCAAAC-3′ | (1186–11671-150) |
| cag15-F | 5′-TTATTTATCTCTGACAAGAG-3′ | (17463–174441-160) |
| cag15-R | 5′-ATGGGGCAGGCATTTTTTAA-3′ | (17084–171051-160) |
| cag13-F | 5′-GCTAGAGAAAAGGCTGTTGC-3′ | (12771–127521-160) |
| cag13-R | 5′-TGGCGTTAATAGTGGCAATA-3′ | (12369–123881-160) |
| cag12-F | 5′-TTATTCCAAATTTAATTTTA-3′ | (11542–115231-160) |
| cag12-R | 5′-ATGGAACTCGGTTTCAATGA-3′ | (11103–111221-160) |
| cag10-F | 5′-ATGGAAGACTTTTTGTATAA-3′ | (9498–94791-160) |
| cag10-R | 5′-TCACAGTTCGCTTGAACCCA-3′ | (7271–72901-160) |
| cag6-7-F | 5′-ATGGCAACATGGAGATGGTT-3′ | (4289–43081-160) |
| cag6-7-R | 5′-TTAGTTTCCTTTTTTTCAG-3′ | (5174–51551-160) |
| cagLEC-F | 5′-ACATTTTGGCTAAATAAACGCTG-3′ | (3920–39421-160) |
| cagLEC-R | 5′-ATCTTTAGTCTCTTTAGC-3′ | (4732–47151-160) |
| IS-F | 5′-CGCCTTGATCGTTTCAGGATTAGC-3′ | (4229–42531-150) |
| IS-R | 5′-CAACCAACCGAAGCAAGCATAATC-3′ | (2596–27191-150) |