Adenovirus mediated gene transfer efficiency and growth inhibitory effect of AdCAp53 on human gastric cancer cell lines
| Cell line | p53 status | β-gal | ED50AdCAlacZ | ED50 AdCAp53 |
|---|---|---|---|---|
| MKN1 | 143 Val to Ala | 92 (7) | >300 | 2.5 (0.2) |
| MKN7 | 278 Pro to Ser | 91 (4) | >300 | 10.7 (3.5) |
| MKN28 | 251 Ile to Leu | 92 (5) | >300 | 99.0 (24) |
| TMK-1 | 173 Val to Met | 90 (2) | >300 | 90.0 (17) |
| MKN45 | Wild type | 95 (3) | >300 | >300 |
Results are expressed as mean (SD) from three independent experiments.
p53 status was detected by DNA sequencing.22 β-gal is the percentage of X-gal positive cells 24 hours after AdCAlacZ infection at a multiplicity of infection (moi) of 30. ED50, moi required for a 50% reduction in viability of untreated cells.