Sequences and homologies of primers used for allele specific PCR
| Primer sequence (5′–3′) | Per cent homology to trypsinogens A–E | |||||
|---|---|---|---|---|---|---|
| A (cationic) | B | C | D | E (anionic) | ||
| Control (forward) | TGGTCATGGCCAGGTCTATGC | 100 | 95 | <60 | 76 | 90 |
| Control (reverse) | ACAGTTAGCAGAGGTAGAGTG | 100 | <60 | <60 | 95 | 95 |
| Mutation specific (reverse) | GGACAGAATTCTCCTCACAGA | Wild type 95, mutation 100 | 95 | 95 | 95 | 100 |