Antibodies used for immunocytochemistry (ICC) and for immunoprecipitation (IP)
| Type | Source | Dilution for ICC | Dilution for IP | |
|---|---|---|---|---|
| Primary antibody to | ||||
| α-SMA synthetic peptide | Monoclonal IgG | Sigma | 1/500 | / |
| Pig desmin | Monoclonal IgG | Boehringer | 1/10 | / |
| Cow GFAP | Rabbit polyclonal IgG | Dako | 1/2000 | / |
| Human collagen type I | Goat polyclonal AP | SBA | / | 1/40 |
| Rat collagen type III | Rabbit polyclonal AS | Schuppanet al 61 | / | 1/75 |
| Human collagen type IV | Goat polyclonal AP | SBA | / | 1/75 |
| Fibronectin | Rabbit polyclonal AS | Calbiochem | / | 1/300 |
| Rat laminin | Rabbit polyclonal IgG | Calbiochem | / | 1/75 |
| Peroxidase labelled antibody to | ||||
| Mouse IgG | Goat polyclonal AP | Amersham | 1/100 | |
| Rabbit IgG | Goat polyclonal AP | Jackson | 1/500 | |
| Goat IgG | Rabbit polyclonal AP | Jackson | 1/100 |
GFAP, glial fibrillary protein; AP, affinity purified; AS, antiserum.
Sigma Chemie, Bornem, Belgium; Boehringer, Mannheim, Germany; Dako, Ghent, Belgium; Southern Biotechnology Associates, Birmingham, Alabama, USA; Calbiochem, La Jolla, California, USA; Amersham, Little Chalfont, UK; Jackson, West Grove, Pennsylvania, USA.