Comparison of the results obtained by the three reference methods and the DNA enzyme immunoassay (DEIA) to detect Helicobacter pylori resistance to macrolide
| Biopsy sample | Resistance determined by | ||||||
|---|---|---|---|---|---|---|---|
| MIC (mg/l) | PCR/RFLP | Sequence analysis3-150 | DEIA | ||||
| WT | 2143C | 2143G | 2144G | ||||
| 1 | >128 | NR | 2143C | - | + | − | − |
| 2 | 64 | NR | WT | + | + | − | − |
| 3 | 16 | NR | WT | + | + | − | − |
| 4 | 163-151 | NR | WT | + | + | − | − |
| 5 | >128 | BbsI | 2143G | − | − | + | − |
| 6 | 32 | BbsI | 2143G | − | − | + | − |
| 7 | 32 | BbsI | 2143G | − | − | + | − |
| 8 | >128 | BbsI | 2143G | − | − | + | − |
| 9 | 64 | BbsI | ND | − | − | + | − |
| 10 | 64 | BbsI | ND | − | − | + | − |
| 11 | >128 | BbsI | ND | − | − | + | − |
| 12 | 32 | BbsI | ND | − | − | + | − |
| 13 | >128 | BbsI + NR | ND | − | + | + | − |
| 14 | >128 | BbsI + NR | ND | − | + | + | − |
| 15 | 64 | BbsI + NR | ND | + | − | + | − |
| 16 | >128 | BbsI + NR | 2143G | + | − | + | − |
| 17 | >128 | BsaI | 2144G | − | − | − | + |
| 18 | 16 | BsaI | 2144G | − | − | − | + |
| 19 | 16 | BsaI | 2144G | − | − | − | + |
| 20 | 8 | BsaI | 2144G | − | − | − | + |
| 21 | 16 | BsaI | 2144G | − | − | − | + |
| 22 | 16 | BsaI | ND | − | − | − | + |
| 23 | 8 | BsaI | ND | − | − | − | + |
| 24 | 32 | BsaI | ND | − | − | − | + |
| 25 | 16 | BsaI | ND | − | − | − | + |
| 26 | 16 | BsaI | ND | − | − | − | + |
| 27 | 16 | BsaI | ND | − | − | − | + |
| 28 | 4 | BsaI | ND | − | − | − | + |
| 29 | 8 | BsaI | ND | − | − | − | + |
| 30 | 4 | BsaI | ND | − | − | − | + |
| 31 | >128 | BsaI | ND | − | − | − | + |
| 32 | 64 | BsaI | 2144G | − | − | − | + |
| 33 | >128 | BsaI + NR | ND | + | − | − | + |
| 34 | 0.015 | NR | ND | + | − | − | − |
| 35 | 0.015 | NR | WT | + | − | − | − |
| 36 | 0.015 | NR | ND | + | − | − | − |
| 37 | 0.007 | NR | WT | + | − | − | − |
| 38 | 0.007 | NR | ND | + | − | − | − |
| 39 | 0.007 | NR | ND | + | − | − | − |
| 40 | 0.015 | NR | ND | + | − | − | − |
| 41 | 0.007 | NR | ND | + | − | − | − |
| 42 | 0.015 | NR | ND | + | − | − | − |
| 43 | 0.06 | NR | ND | + | − | − | − |
| 44 | 0.015 | NR | ND | + | − | − | − |
| 45 | 0.06 | NR | ND | + | − | − | − |
| 46 | 0.0035 | NR | WT | + | − | − | − |
| 47 | 0.007 | NR | WT | + | − | − | − |
MIC was determined by the agar dilution method.BsaI, PCR product restricted byBsaI; BbsI, PCR product restricted by BbsI. +, OD450 value higher than the cut off value; −, OD450 value lower than the cut off value.
↵3-150 Sequence analysis of the 23S rRNA gene revealed the genotype at residues 2143 and 2144.
↵3-151 MIC tested by Etest: 0.047 mg/l and presence of resistant mutants.
MIC, minimal inhibition concentration; PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism; WT, wild type; ND, not done; NR, not restricted.