Effect of BFT-2 on electrophysiology of human colonic mucosa
| BFT-2 | ||||||
|---|---|---|---|---|---|---|
| Controls (n=8) | Luminal 10 nM (n=6) | Serosal 10 nM (n=7) | Serosal 5 nM (n=6) | Serosal 2.5 nM (n=5) | Serosal 1.25 nM (n=5) | |
| PD (mV) | ||||||
| Baseline | −10.8 (1.8) | −10.2 (0.5) | −10.9 (1.0) | −9.6 (1.6) | −9.5 (1.1) | −9.8 (1.2) |
| 1 h | −10.0 (1.5) | −10.1 (0.8) | −9.4 (1.5) | −10.1 (0.9) | −9.4 (0.9) | −10.3 (1.5) |
| 4 h | −9.8 (1.5) | −6.8 (1.0)1-150 † | −1.7 (0.7)1-150 † | −4.6 (0.9)1-150 † | −7.1 (0.5)1-150 † | −10.4 (1.1) |
| Isc (μA/cm2) | ||||||
| Baseline | 97 (5) | 97 (11) | 98 (10) | 92 (10) | 89 (4) | 93 (7) |
| 1 h | 94 (8) | 96 (10) | 93 (13) | 95 (8) | 93 (7) | 98 (11) |
| 4 h | 95 (7) | 79 (5)1-150 † | 43 (11)1-150 † | 69 (9)1-150 † | 77 (5)1-150 † | 97 (9) |
| R (Ohm.cm2) | ||||||
| Baseline | 108 (6) | 105 (7) | 106 (6) | 104 (6) | 106 (6) | 104 (8) |
| 1 h | 104 (10) | 104 (7) | 100 (10) | 105 (6) | 103 (5) | 106 (10) |
| 4 h | 103 (8) | 85 (4)1-150 † | 38 (7)1-150 † | 64 (10)1-150 † | 88 (5)1-150 † | 102 (7) |
Results expressed as mean (SEM); n = number of experiments.
↵1-150 p<0.05 versus controls; †p<0.05 compared with baseline obtained in the same tissue before exposure to toxin.