Review of cytokine analysis of gastric mucosal biopsy specimens of Helicobacter pylori infected and uninfected gastritic and histologically normal patients
| Referencea | ||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 123 | 121 | 122 | 103 | 125 | 127 | 124 | 126 | 122 | 85 | 122 | 131 | 84 | 128 | |||||||||||||
| RT-PCR (% positive samples) | IHC (% positive samples (median MNC/mm2)) | ESP (median no spots) | FACS (median positive cells) | ELISA (median pg/ml) | ELISA (median values) | |||||||||||||||||||||
| Whole biopsy | Biopsy MNC | Whole biopsy | LPL & IEL | CD4+ LPL & IEL | CD3+ LPL | Whole biopsy | Culture supernatant | |||||||||||||||||||
| IFN-γ | ||||||||||||||||||||||||||
| Hp+ IGM | 83b | 12b | 100 (PU) | 85b | 90% PU (5)** 90% AS (6) | 1452-150 | 35 | 86 | 91 | |||||||||||||||||
| Hp− IGM | 100b | 13 | 0 (NUD) | 75 | 371† | |||||||||||||||||||||
| NGM | 83 | 71 | 10% (0.1) | 20 | 64 | |||||||||||||||||||||
| IL-12 | ||||||||||||||||||||||||||
| Hp+ IGM | 100 | 100 | 78§§ § | 34b | ||||||||||||||||||||||
| Hp− IGM | 0 | 55 | ||||||||||||||||||||||||
| NGM | 33 | 43 | 42 | |||||||||||||||||||||||
| IL-2 | ||||||||||||||||||||||||||
| Hp+ IGM | 0 | 53‡‡ | 91 | 19b ng/mg | ||||||||||||||||||||||
| Hp− IGM | 0 | 13 | 19.5 ng/mg | |||||||||||||||||||||||
| NGM | 0 | |||||||||||||||||||||||||
| IL-4 | ||||||||||||||||||||||||||
| Hp+ IGM | 0 | 0 | 90% PU (4.5) b 90% AS (3) | 90c | 0 | 5 | <2 | 8.4 ng/mg‡ | ||||||||||||||||||
| Hp− IGM | 0 | 0 | 5.4 ng/mg | |||||||||||||||||||||||
| NGM | 0 | 80% (1) | 40 | 5 | ||||||||||||||||||||||
| IL-10 | ||||||||||||||||||||||||||
| Hp+ IGM | 22§ | 55¶ | 80 | 85†† | 0.2b | 411 pg/g/24 h‡‡ | ||||||||||||||||||||
| Hp− IGM | 13b | 31 | 58 | 78 | ||||||||||||||||||||||
| NGM | 0 | 100 | 100 | 0.7 | 75 | |||||||||||||||||||||
| IL-6 | ||||||||||||||||||||||||||
| Hp+ IGM | 17b | 25††† | 90% PU (5)‡‡ ‡ 100% AS (5) | 106 pg/ml§§ | 16.2 ng/mg¶¶ | |||||||||||||||||||||
| Hp− IGM | 0b | 0 | 8.25 mg/ng | |||||||||||||||||||||||
| NGM | 0 | 50% (0.1) | 25 pg/ml | |||||||||||||||||||||||
| TGF-β | 0 | 100% PU (9)‡‡ ‡ | ||||||||||||||||||||||||
| Hp+ IGM | 100% AS (10) | |||||||||||||||||||||||||
| Hp− IGM | 0 | |||||||||||||||||||||||||
| NGM | 0 | 100% (3) | ||||||||||||||||||||||||
↵2-150 Significantly different from H pylori negative (Hp−) inflamed gastric mucosae (IGM) (p<0.05) and normal gastric mucosae (NGM) (p<0.05). **Peptic ulcer (PU) and asymptomatic (AS) combined significantly different from NGM (p<0.01) but not each other. †Significantly different from NGM (p<0.01).‡Significantly different from Hp− IGM (p=0.01). §Significantly different from uninfected IGM (p=0.06). ¶Trend towards significant difference from uninfected IGM (p=0.11). ††Trend towards significant difference from uninfected IGM (p=0.08). ‡‡Significantly different from NGM (p<0.01) and Hp− IGM (p<0.05). †††Significantly different from Hp− IGM (p=0.06). ‡‡ ‡PU and AS combined significantly different from NGM (p<0.05) but not from each other.§§Significantly different from NGM (p<0.001). ¶¶Significantly different from Hp− IGM (p<0.001). §§ §Significantly different from NGM and Hp− IGM grouped together (p=0.054).
aWhere unnoted, no comparative statistics were performed. bNot significantly different from NGM and/or uninfected IGM. c H pyloripositive (Hp+) and Hp− IGM combined, and not significantly different from NGM.
IFN, interferon; TGF, transforming growth factor; RT-PCR, reverse transcription polymerase chain reaction; IEL, intraepithelial lymphocytes; IHC; immunohistochemistry; LPL, lamina propria lymphocytes; ESP, ELISPOT; ELISA, enzyme linked immunosorbent assay; MNC, mononuclear cells.