Patient | Specimen | Date | Codon 528 | Codon 552 |
Patient 1 | #1 | 09/09/94 | Leucine | Methionine |
#2 | 11/07/95 | Methionine | Valine | |
#3 | 08/12/95 | Methionine | Valine | |
#4 | 18/03/96 | Methionine | Valine | |
#5 | 16/05/96 | Methionine | Valine | |
#6 | 24/07/96 | Methionine | Valine | |
#7 | 04/09/96 | Leucine | Methionine | |
Patient 2 | #8 | 04/08/95 | Leucine | Methionine |
#9 | 19/09/96 | Methionine | Valine | |
#10 | 18/03/97 | Methionine | Valine | |
#11 | 19/05/97 | Leucine and methionine | Methionine, valine, and isoleucine1-150 | |
Patient 3 | #12 | 20/03/96 | Leucine | Methionine |
#13 | 28/05/97 | Leucine | Isoleucine | |
Patient 4 | #14 | 19/12/94 | Leucine | Methionine |
#15 | 17/10/95 | Leucine | Isoleucine | |
#16 | 12/12/95 | Leucine | Isoleucine | |
#17 | 26/06/96 | Leucine | Methionine | |
#18 | 04/08/96 | Leucine | Methionine | |
#19 | 04/06/97 | Leucine | Methionine |
Part of the polymerase gene (nt 446–863) was amplified by PCR and directly sequenced using the same primers and an automatic sequencer. Multiple serum samples were examined (#1 to #19) and are referred to in the text and figures. Multiple changes were observed, and the amino acids at polymerase codon positions 528 and 552 are shown.
↵1-150 Direct sequencing of serum #11 suggested that a mixture of viral species was present—leucine or methionine at position 528, and methionine and valine or isoleucine at 552.