Species Molecular weight (kDa) |
3H-PEG400 cm/h 0.4 |
Microperoxidase cm/h 1.8 |
3H-PEG4000 cm/h 4 |
HRP
cm/h 40 |
Hamster
PrPsc
cm/h 28 |
Ovine | 0.09 (0.01) | 0.086 (0.015) | 0.01 (0.001) | 0.0027 (0.0005) | 0.0037 (0.001) |
(n=24) | (n=15) | (n=33, 5 animals) | (n=37, 5 animals) | (n=6, 2 animals) | |
Bovine | 0.28 (0.02)** | 0.14 (0.02)1-150 | 0.16 (0.05)** | 0.019 (0.0015)*** | 0.026 (0.005)** |
(n=23) | (n=24) | (n=32, 5 animals) | (n=30, 4 animals) | (n=17, 3 animals) | |
Probe hydrated radius (nm) | 0.5 | 0.7 | 1.2 | 3.0 | 2.5 |
Ratio bovine:ovine | 3.1 (0.13) | 1.64 (0.17) | 10.67 (0.2)1-151 | 7.03 (0.20)1-151 | 7.02 (0.33)1-151 |
Results expressed as mean (SEM). The number in parentheses is the number of individual tissues on which flux measurements were made.
↵1-150 p<0.05, **p<0.025, ***p<0.001, bovine significantly greater than ovine.
↵1-151 p<0.001, Student's two tailed t test, bovine:ovine flux ratio of horseradish peroxidase (HRP) activity and125I-scrapie prion protein (PrPsc) exceed that of PEG400 and microperoxidase. The molecular radii of the probes were obtained from Matsukawa et al,37Taylor et al,29 and Naftalin and Pedley.44