Quantitative histological examination and ELISA of isolated vascularly perfused rat colon after luminal administration of short chain fatty acids
|
Percent of cavitated mucus cells | Number of mucus cells/crypt | n |
Mucin (μg/mg DNA) | n | |
| Control group | 11.9 (2.8) | 30.1 (1.5) | 7 | 144.7 (13.4) | 9 |
| Butyrate 5 mM | 26.1 (2.0)3-150 | 29.5 (1.2) | 11 | 338.1 (63.6)3-150 | 9 |
| Butyrate 100 mM | 9.8 (0.7) | 31.3 (0.6) | 6 | 164.6 (17.7) | 6 |
| Propionate 5 mM | 16.7 (2.3) | 31.2 (1.4) | 5 | 190.3 (28.3) | 6 |
| Propionate 100 mM | 16.6 (4.8) | 28.4 (1.0) | 5 | 215.3 (30.1) | 6 |
| Acetate 5 mM | 16.2 (5.3) | 32.5 (1.1) | 6 | 176.9 (40.2) | 6 |
| Acetate 100 mM | 26.9 (4.8)3-150 | 32.4 (0.7) | 8 | 381.3 (50.6)3-150 | 6 |
Values are mean (SE); n = no of animals. Cavitation of mucus cells is the hallmark of accelerated mucus discharge by a process of compound exocytosis.
↵3-150 p<0.05 compared with control group.