Interleukin (IL) 1 receptor antagonist (ra), IL-8, IL-1β, and IL-6 content of freshly isolated intestinal epithelial cells (IECs) and lamina propria mononuclear cells (LPMNCs) normalised to cell protein
| IL-1ra | IL-8 | IL-1β | IL-6 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IEC | LPMNC | IEC | LPMNC | IEC | LPMNC | IEC | LPMNC | ||||||||
| CO (0) | 7397 (1039) | 3199 (375) | 137 (19) | 1144 (145) | 1.8 (0.6) | 97 (14) | 1.4 (0.2) | 42 (5.6) | |||||||
| CO (1–3) | 8973 (1570) | 4325 (380) | 187 (17) | 1836 (133) | 9.8 (2.1) | 263 (46) | 2.9 (1.3) | 100 (18) | |||||||
| CD (0) | 5706 (1056) | 3836 (154) | 122 (8.7) | 1853 (436) | 9.9 (2.9) | 305 (45) | 3.1 (1.0) | 86 (1.9) | |||||||
| CD (1–3) | 9301 (2188) | 5440 (748) | 216 (29) | 2005 (194) | 30 (4.4) | 348 (80) | 8.8 (1.6) | 185 (34) | |||||||
| UC (0) | 5914 (1777) | 4253 (533) | 181 (69) | 2283 (197) | 11 (5.9) | 339 (39) | 13 (1.6) | 99 (12) | |||||||
| UC (1–3) | 17856 (2013) | 10216 (348) | 1162(206) | 6624 (1812) | 29 (6.8) | 697 (24) | 17 (2.2) | 422 (32) | |||||||
Cytokines were measured in homogenised, freshly isolated IECs from 50 control patients (CO, 0), five patients with unspecific intestinal inflammation (CO, 1–3), 24 patients with Crohn's disease (CD), and 14 patients with ulcerative colitis (UC). Cytokines were also measured in freshly isolated LPMNCs from 17 control patients, five patients with unspecific intestinal inflammation, nine patients with Crohn's disease, and nine patients with ulcerative colitis.
Data (mean (SEM) are expressed as pg cytokine/mg total protein.
0, mucosa not inflamed; 1, slightly inflamed; 2, moderately inflamed; 3, severely inflamed.