Generation of reactive oxygen species by different faecal preparations
| Sample | 2,5-DHBA (μM) | 2,3-DHBA (μM) |
Catechol (μM) | Total (μM) |
| HX/XO | 122 (0.7)1-150 | 143 (2.0)1-150 | 35 (0)1-150 | 299 (3)1-150 |
| Faeces, wet (100 mg)1-150 | 39 (14) | 47 (16) | – | 87 (30) |
| Faeces, wet (100 mg)1-151 | 9.6 (3.5) | 12.9 (4.9) | – | 22.4 (8.4) |
| Faeces, wet (100 mg)1-152 | 47 (49) | 58 (65) | – | 105 (114) |
| Faeces, freeze dried (25 mg)1-153 | 27 (9) | 34 (15) | – | 61 (24) |
Experiments were conducted at 37°C as described in Materials and methods. Values are mean (SD)
↵1-150 Phosphate buffer, pH = 6.50.
↵1-151 Tris buffer, pH = 6.50.
↵1-152 Phosphate buffer, pH = 6.50. Filter-sterilised as described in Materials and methods.
↵1-153 Phosphate buffer, pH = 6.50. Representing on average the equivalent wet weight.
HX/XO, Hypoxanthine/xanthine oxidase; 2,5-DHBA, 2,5-dihydroxybenzoic acid; 2,3-DHBA, 2,3-dihydroxybenzoic acid.