Table 2

Glycosyltransferase primer sequences

Glycosyltransferase	Primer sequence	Amplicon length (bp)	Reference
FT-I forward	TAT TCC GCA TCA CCC TGC	173	38
FT-I reverse	CTG TTC CCG GAG ATG GTG

FT-III/V forward	CTG CTG GTG GCT GTG TGT TTC TTC TCC TAC	447/486	49
FT-III/V reverse	CAG CCA GCC GTA GGG CGT GAA GAT GTC GGA

FT-IV forward	GGT GCC CGA AAT TGG GCT CCT GCA CAC	319	49
FT-IV reverse	CCA GAA GGA GGT GAT GTG GAC AGC GTA

FT-VI forward	CTC AAG ACG ATC CCA CTG TGT AC	404	49
FT-VI reverse	CAG CCA GCC GTA GGG CGT GAA GAT GTC GGA

FT-VII forward	CTC GGA CAT CTT TGT GCC CTA TG	288	45
FT-VII reverse	CGC CAG AAT TTC TCC GTA ATG TAG

ST3Gal-I forward	ATG AGG TGG ACT TGT ACG GC	253	50
ST3Gal-I reverse	AAC GGC TCC AGC AAG ATG

ST3Gal-III forward	AAC AAG TCT CTG GGG TCA CG	307	51
ST3Gal-III reverse	TGA GGA TTC GAA TCT CAG GG

ST3Gal-IV forward	CTT CTT CAT GGA GAT TGC AGC	320	52
ST3Gal-IV reverse	CTA CAG CTC TTG CCC AGG TC

ST6Gal-I forward	AAA AAC CTT ATC CCT AGG CTG C	379	53
ST6Gal-I reverse	TGG TAG TTT TTG TGC CCA CA

β1,4 GalT forward	TCT ATG TTA TCA ACC AGG CGG	392	54
β1,4 GalT reverse	GAT CAT GCG ACA CCT CCC

GNT-V forward	GTG GAT AGC TTC TGG AAG AA	856	55
GNT-V reverse	CAG TCT TTG CAG AGA GCC

β-actin forward	GGC ATC GTG ATG GAC TCC G	622	56
β-actin reverse	GCT GGA AGG TGG ACA GCG A

Primers for FT-III/V, FT-IV and FT-VI were those published by Yago et al.49 All other primers were designed with the HUSAR software according to published glycosyltransferase sequences (references listed in the last column). Amplicons obtained were sequenced and compared with published data.