Super u-PA (ng/mg protein) | Cellular u-PA (ng/mg protein) | Cellular u-PAR (ng/mg protein) | Cellular u-PA activity (mIU/mg protein) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Level | % Change | Level | % Change | Level | % Change | Level | % Change | ||||
LIM1215 | |||||||||||
Basal | 0.71 (0.05) | — | 0.69 (0.11) | — | 1.01 (0.2) | — | 479 (45) | — | |||
Butyrate | 0.46 (0.03) | −30 (5)1-a | 0.52 (0.11) | −35 (4)1-a | 3.78 (0.73) | 289 (74)1-c | 97 (9) | −79 (2)d | |||
EGF | 2.17 (0.16) | 214 (38)1-a | NT 1-b | NT | 3.89 (0.21) | 334 (54)1-a | 925 (80) | 189 (24)a | |||
PMA | 2.33 (0.25) | 267 (60)1-a | NT | NT | 4.74 (0.31) | 425 (57)1-a | 990 (99) | 245 (67)a | |||
Caco-2 | |||||||||||
Basal | 0.2 (0.06) | — | 0.05 (0.02) | — | 0.15 (0.02) | — | 184 (12) | — | |||
Butyrate | 0.35 (0.07) | 68 (6)1-a | 0.13 (0.01) | 172 (38)1-c | 0.35 (0.04) | 137 (17)1-d | 417 (42) | 125 (17)d |
Supernatant (Super) u-PA, expression of cellular u-PA and u-PAR, and cell associated u-PA activity were assayed in wounded LIM1215 and Caco-2 monolayers after a 24 hour incubation with and without exposure to 2 mM butyrate, 20 ng/ml EGF and 10 nM PMA. Values are mean (SEM) of 3–6 experiments.
↵1-a p<0.05;
↵1-b not tested;
↵1-c p<0.01;
↵1-d p<0.001. All p values are expressed relative to basal levels and were determined using a paired ttest.