Table 2

Characterisation of the u-PA and u-PAR oligonucleotides (ONs)

Supernatant u-PACellular u-PARu-PA stim. migrationu-PA stim.3H thy. uptake
LIM1215Caco-2LIM1215Caco-2LIM1215Caco-2LIM1215
u-PA antisense85 (7)a b 822-d NTNTNT
u-PA sense 3 (4)2-a  52-d NTNTNT
u-PAR antisenseNT2-c 82 (5)a b 782-d 77 (8)2-e 68 (12)ef 71 (13)e f
u-PAR senseNT 2 (6)2-a  42-d  5 (2)2-e  1 (6)2-e  8 (5)2-e

  • Effects of 48 hour exposure to u-PA and/or u-PAR antisense and sense ONs (20 mM) on supernatant u-PA levels, cellular u-PAR expression, and 100 ng/ml u-PA stimulated (stim.) migration and3H thymidine (thy.) uptake in wounded LIM1215 and Caco-2 monolayers. Values are the results of 2–4 experiments.

  • ↵2-a Percentage inhibition (mean (SEM)) compared with controls;

  • 2-b p<0.01 relative to control, pairedt test;

  • ↵2-c not tested;

  • ↵2-d percentage inhibition (mean of two experiments) compared with controls;

  • ↵2-e percentage inhibition (mean (SEM)) of stimulatory effect;

  • 2-f p<0.01 relative to stimulatory effect alone, paired t test.