Supernatant u-PA | Cellular u-PAR | u-PA stim. migration | u-PA stim.3H thy. uptake | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
LIM1215 | Caco-2 | LIM1215 | Caco-2 | LIM1215 | Caco-2 | LIM1215 | |||||||
u-PA antisense | 85 (7)a b | 822-d | NT | NT | NT | ||||||||
u-PA sense | 3 (4)2-a | 52-d | NT | NT | NT | ||||||||
u-PAR antisense | NT2-c | 82 (5)a b | 782-d | 77 (8)2-e | 68 (12)ef | 71 (13)e f | |||||||
u-PAR sense | NT | 2 (6)2-a | 42-d | 5 (2)2-e | 1 (6)2-e | 8 (5)2-e |
Effects of 48 hour exposure to u-PA and/or u-PAR antisense and sense ONs (20 mM) on supernatant u-PA levels, cellular u-PAR expression, and 100 ng/ml u-PA stimulated (stim.) migration and3H thymidine (thy.) uptake in wounded LIM1215 and Caco-2 monolayers. Values are the results of 2–4 experiments.
↵2-a Percentage inhibition (mean (SEM)) compared with controls;
2-b p<0.01 relative to control, pairedt test;
↵2-c not tested;
↵2-d percentage inhibition (mean of two experiments) compared with controls;
↵2-e percentage inhibition (mean (SEM)) of stimulatory effect;
2-f p<0.01 relative to stimulatory effect alone, paired t test.