Synthetic oligonucleotides and experimental conditions used in reverse transcription-polymerase chain reaction (RT-PCR) analysis
| Gene | Position | Fragment size (bp) | Sequence | Cycling number | Hybridisation (°C) |
| β-actin | 326–351 | 380 | 5′-ATATCGCTGCGCTCGTCGTCGACAA | 28 | 50 |
| 733–706 | 3′-TACATGCATCGGTAGGTCCGACACAA | ||||
| Ribosomal | 340–360 | 486 | 5′-GTTCACCAAGGAGGACCTCA | 23–25 | 50 |
| phosphoprotein | 847–828 | 3′-AGACACCTCTGCCTAATGTG | |||
| TGF-β1 | 731–752 | 661 | 5′-GAAGTCACCCGCGTGCTAATGG | 30–32 | 50 |
| 1415–1392 | 3′-GGATGTAAACCTCGGACCTGTGTG | ||||
| TGF-β2 | 1–22 | 192 | 5′-TTCGCAGGTATCGATGGCACCT | 30–40 | 50 |
| 214–192 | 3′-CGTCGTATTAACGACGGAAGCGG | ||||
| TNF-α | 3033–3055 | 122 | 5′-TTCTGTGAAAACGGAGCTAAAC | 35–40 | 50 |
| 3178–3155 | 3′-TTTATTACGACTAAACCACTGGT | ||||
| TGF-α | 3538–3557 | 373 | 5′-ATGTTGTTCCCTGCAAGTCC | 25–30 | 50 |
| 3930–3911 | 3′-ACTATGGAGAGGGGTCGCTT | ||||
| IL-1β | 823–843 | 169 | 5′-GCAATGGTCGGGACATAGTT | 33 | 50 |
| 1011–992 | 3′-GAATGTGCCACGGTTTTCTT | ||||
| SI | 321–344 | 432 | 5′-TGGGATGATGGCCAATCTAAAGAT | 32 | 60 |
| 776–753 | 3′-ATAGGGATTGTGTTCTGGAGGAAA |