Primers used to detect house keeping gene GAPDH, leptin, and different splice variants of the leptin receptor gene
| Primer name | Sequence 5′—3′ | Length | Product size |
| GAPDH forward | ggT•Cgg•agT•Caa•Cgg•aTT•Tgg•TCg | 24 | |
| GAPDH reverse | CCT•CCg•aCg•CCT•gCT•TCa•CCa•C | 22 | 782 |
| Leptin forward | CCa•aga•Tgg•aCC•aga•CaC•Tg | 20 | |
| Leptin reverse | gCC•aCC•aCC•TCT•gTg•gag•Ta | 20 | 220 |
| Leptin receptor forward | TTg•TgC•Cag•Taa•TTa•TTT•CCT•CTT | 24 | |
| Reverse primer: universal | aTa•gCT•TTT•TCa•TTC•TTT•ggT•gTg | 24 | 78 |
| Reverse primer: huB219.1 | CTg•Tgg•CCT•TCC•gCa•gTg | 18 | 227 |
| Reverse primer: huB219.2 | aCC•TCC•aCC•Cag•Tag•TTC•CTT | 21 | 176 |
| Reverse primer: huB219.3 | agT•Tgg•CaC•aTT•ggg•TTC•aT | 20 | 200 |
| Reverse primer: huOB-R | CTg•aTC•agC•gTg•gCg•TaT•TT | 20 | 439 |