Michelis-Menten constants, maximum rates of retinoic acid formation and their coefficients in the cytosol obtained from the liver of rats (n=11) at different ethanol concentrations, calculated using retinol and retinal as substrates
| Substrate | Ethanol concentration (%) (mM) | Vmax (pmol/min/mg) | Km(μM) | Vmax/Km (ml/min/g) |
|---|---|---|---|---|
| 0 | 28.1 (2.1) | 18.8 (5.1) | 1.49 | |
| Retinol | 0.05 (8.6) | 11.2 (0.3) | 34.4 (2.6) | 0.33 |
| 0.1 (17) | 9.8 (0.5) | 43.4 (7.1) | 0.23 | |
| 0.2 (34) | 7.4 (0.6) | 33.8 (8.7) | 0.22 | |
| 0 | 31.5 (3.3) | 2.20 (0.7) | 14.3 | |
| Retinal | 0.05 (8.6) | 12.7 (0.5) | 1.19 (0.1) | 10.7 |
| 0.1 (17) | 11.2 (0.9) | 1.12 (0.2) | 10.0 | |
| 0.2 (34) | 8.1 (1.0) | 0.67 (0.2) | 12.2 |