Nuclei densities after immunostaining with anti-5-MeCyd antibodies in colon biopsies. Paraffin embedded sections from paired tissues were processed as described in methods. Numbers indicate average optical density (mean (2SD)) for each sample
| Case No | Location | Age | Dukes' stage | Differentiation | OD tumour | OD normal | Difference |
|---|---|---|---|---|---|---|---|
| 1 | Right colon | 84 | D | WD | 0.796 (0.039) | 1.014 (0.039) | 21.4% |
| 2 | Right colon | 63 | D | WD | 0.766 (0.013) | 0.997 (0.026) | 23.2% |
| 3 | Right colon | 75 | D | MD | 0.816 (0.041) | 1.025 (0.035) | 20.0% |
| 4 | Right colon | 85 | C | MD | 0.773 (0.019) | 1.021 (0.046) | 24.3% |
| 5 | Right colon | 69 | D | WD | 0.792 (0.041) | 1.030 (0.038) | 23.0% |
| 6 | Left colon | 50 | B | MD | 0.764 (0.033) | 1.019 (0.048) | 25.1% |
| 7 | Left colon | 58 | C | WD | 0.739 (0.051) | 1.021 (0.031) | 27.6% |
| 8 | Left colon | 64 | C | PD | 0.751 (0.039) | 1.018 (0.021) | 26.2% |
| 9 | Left colon | 60 | B | WD | 0.723 (0.026) | 1.026 (0.026) | 29.5% |
| 10 | Left colon | 77 | B | WD | 0.837 (0.037) | 1.010 (0.032) | 17.1% |
| 11 | Left colon | 74 | A | WD | 0.784 (0.043) | 1.006 (0.024) | 22.1% |
| 12 | Left colon | 91 | B | WD | 0.818 (0.055) | 1.027 (0.039) | 20.4% |
| 13 | Left colon | 62 | C | MD | 0.787 (0.480) | 1.009 (0.032) | 22.0% |
OD, optical density; WD, well differentiated; MD, moderately differentiated; PD, poorly differentiated.
Dukes classification: Dukes CE. The classification was established according to J Pathol Bacteriol1932;35:323–44.