Case | Stage | Grade | Codon | Mutation | Amino acid substitution | p21rasIHC3-a | p16 methylationb | p16 IHC |
Codon 12 | GGT (wild type) | Glycine | ||||||
1 | II | 1 | 12 | GGT→AGT | Serine | ++ | ++ | — |
2 | IIIA | 2 | 12 | GGT→GAT | Aspartate | ++ | ++ | — |
3 | IIIA | 2 | 12 | GGT→GTT | Valine | ++ | ++ | — |
4 | IIIB | 3 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
7 | IIIA | 2 | 12 | GGT→GCT | Alanine | + | ++ | — |
8 | IVA | 1 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
13 | IIIA | 1 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
14 | IVA | 3 | 12 | GGT→GCT | Alanine | + | ++ | — |
17 | IIIA | 2 | 12 | GGT→GAT | Aspartate | ++ | ++ | — |
25 | IIIB | 2 | 12 | GGT→GCT | Alanine | + | ++ | — |
28 | IIIA | 2 | 12 | GGT→GTT | Valine | ++ | ++ | — |
29 | IVA | 2 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
31 | IIIA | 2 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
33 | IVA | 2 | 12 | GGT→GAT | Aspartate | ++ | ++ | — |
35 | IIIA | 1 | 12 | GGT→TGT | Cysteine | ++ | ++ | — |
39 | IIIA | 2 | 12 | GGT→AGT | Serine | +++ | ++ | — |
40 | II | 1 | 12 | GGT→GTT | Valine | ++ | ++ | — |
Codon 13 | GGC (wild type) | Glycine | ||||||
6 | IIIA | 1 | 13 | GGC→GAC | Aspartate | +++ | ++ | — |
12 | I | 1 | 13 | GGC→TGC | Cysteine | ++ | ++ | — |
21 | IVA | 1 | 13 | GGC→CAT | Aspartate | + | ++ | — |
27 | IVA | 2 | 13 | GGC→TGC | Cysteine | ++ | ++ | — |
34 | IIIA | 1 | 13 | GGC→GAC | Aspartate | +++ | ++ | — |
5 non-neoplastic tissue | 12 | GGT→GAT | Aspartate | + | — | ++ | ||
11 non-neoplastic tissue | 13 | GGC→GAC | Aspartate | ++ | — | ++ |
↵3-a IHC, defined by immunohistochemistry;
3-b methylation status defined by methylation specific polymerase chain reaction.