Results for expression of the secreted mucin genes (MUC2, MUC5AC, MUC5B, and MUC6)
| MUC2 | MUC5AC | MUC5B | MUC6 | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Histological diagnosis | n | Age | M:F | S | G | Gl | S | G | Gl | S | G | Gl | S | G | Gl | |||
| In situ hybridisation | ||||||||||||||||||
| Normal oesophagus | 8 | 73 | 5:3 | − | − | − | − | − | − | − | − | +++ (8/8) | − | − | ++ (1/8) | |||
| Gastric metaplasia | 8 | 57 | 4:4 | − | − | − | +++ (8/8) | − | ++ (8/8) | − | − | − | − | − | +++ (7/8) | |||
| Barrett's oesophagus | 18 | 68 | 11:7 | − | +++ (18/18) | − | +++ (17/18) | − | + (17/18) | − | − | − | − | − | +++ (18/18) | |||
| Dysplastic Barrett's oesophagus | 6 | 72 | 4:2 | + (1/6) | + (4/6) | − | + (6/6) | − | + (6/6) | − | − | − | − | − | + (5/6) | |||
| Adenocarcinoma | 6 | 72 | 5:1 | − | − | − | − | − | − | − | − | − | + (3/6) | − | − | |||
| Immunohistochemistry | ||||||||||||||||||
| Normal oesophagus | 8 | 73 | 5:3 | − | − | − | − | − | − | − | − | +++ (8/8) | − | − | ++ (1/8) | |||
| Gastric metaplasia | 8 | 57 | 4:4 | − | − | − | +++ (8/8) | − | ++ (8/8) | − | − | − | − | − | +++ (8/8) | |||
| Barrett's oesophagus | 18 | 68 | 11:7 | − | +++ (18/18) | − | +++ (18/18) | − | ++ (18/18) | − | − | − | − | − | +++ (17/18) | |||
| Dysplastic Barrett's oesophagus | 6 | 72 | 4:2 | + (1/6) | + (3/6) | − | + (6/6) | − | + (6/6) | − | − | − | − | − | + (5/6) | |||
| Adenocarcinoma | 6 | 72 | 5:1 | − | − | − | − | − | − | − | − | − | + (3/6) | − | − | |||
S, superficial cell layer; G, goblet cells; Gl, deep glands or crypts.
Scoring: average intensity of positively labelled cells: +++ strong; ++ moderate; + weak; − absent.
Values in parentheses denote the number of positively labelled specimens.