Human mucin and trefoil peptide antibodies.Characteristics of antibodies and dilutions used for immunohistochemical methodologies
| Antibody | Epitope | Dilutions | Reference | |
|---|---|---|---|---|
| Mucin | ||||
| MUC2 | LUM2–3 | C terminal end of non-VNTR domain | 1:3000 | Hovenberg et al 44 |
| MUC5AC | LUM5–1 | C terminal region, and tandem repeat region flanking domain | 1:800 | Hovenberget al 46 |
| MUC5B | MAN-5B1 | Non-VNTR domain, low glycosylated region | 1:2500 | Thornton et al 45 |
| MUC6 | GPEP24 | VNTR domain, glycosylation dependent region | 1:1000 | 1-150 |
| Trefoil | ||||
| TFF1 | pS2 (GE2) 28AA | C terminal domain | 1:2000 | Rio et al 47 |
| TFF2 | Anti-hSP | C terminal domain | 1:5 | Elia et al 48 |
| TFF3 | HM:169 | C terminal domain | 1:500 | Podolskyet al 40 |
VNTR, variable number tandem repeat.
↵1-150 Personal communication: Dr I Carstedt, Lund, Sweden.