Summary of polymerase chain reaction (PCR) studies performed to identify measles virus in inflammatory bowel disease
| Study group | Diagostic groups | Tissue source | Experimental procedure | Assay sensitivity | Positive control | Measles virus presence | ||||||
| Royal Free Hospital, London, UK35 | CD, UC, IC, controls | Resected material + PBMC | Hybrid capture + N, H gene specific RT-PCR | 104 RNA molecules | SSPE (brain); vaccine recipient + SSPE (PBMC) | −ve | ||||||
| NIBSC, UK20 | CD, UC, IC, controls | Biopsies + lymphocytes | N gene specific RT-PCR nested PCR | 5.5×10-3 pfu | SSPE (brain); wild-type measles virus strain (94/31825) in Vero cells | −ve | ||||||
| Hirosaki University, Japan33 | CD, UC, controls | Resected intestine | N, F gene specific RT-PCR nested PCR | Single viral genomic RNA | SSPE (brain); Vero E6 cells infected with Toyoshima strain of measles virus | −ve | ||||||
| Akita and Osaka University, Japan32 | CD, UC, controls | Resected intestine + biopsies | N, M, F, H gene specific RT-PCR | Not reported | Not reported | −ve | ||||||
| Tokyo Medical University, Kitasato Institute Tokyo, Japan; Royal Free Hospital, London, UK37 | CD, UC, controls | PBMC | F, H gene specific RT-PCR nested PCR | Not reported | SSPE (brain); SSPE (PBMC), vaccine and wild strains | +ve | ||||||
| NIBSC, UK34 | CD (affected, unaffected, lymph nodes) | Resection specimen (affected and unaffected intestine lymph nodes) | N, M, H gene specific RT-PCR nested PCR | N: 5.5×10−2– 5.5×10−3 pfu | SSPE (brain); wild-type measles virus strain (94/31825) in Vero cells | −ve | ||||||
| M: 5.5×10−3 pfu | ||||||||||||
| H: 5.5 ×10−1–5.5 ×10−2 pfu | ||||||||||||
CD, Crohn's disease; UC, ulcerative colitis; IC, indeterminate colitis; pfu, plaque forming unit; PBMC, peripheral blood mononuclear cells; N, nucleocapsid protein gene; M, matrix protein gene; F, fusion protein gene; H, haemagglutinin protein gene.