Primers and reaction conditions used in polymerase chain reaction amplifications
| Gene | Oligonucleotide sequences | Product size (bp) | Cycles | Reaction conditions | ||||
| IL-1α | Sense: acaggtagtgagacccacct | 389 | 30 | 94°C 5 min, 94°C 1 min, 58°C 1 min, 72°C 2 min, 72°C 10 min | ||||
| Antisense: tagtgagcaaactatagggt | ||||||||
| IL-1β | Sense: gtggcagctacctatgtctt | 506 | 24 | 94°C 5 min, 94°C 1 min, 60°C 1 min 30, 72°C 2 min, 72°C 10 min | ||||
| Antisense: gagaggtgctgatgtaccag | ||||||||
| TGF-β1 | Sense: ggactctccacctgcaagac | 392 | 30 | 94°C 5 min, 94°C 1 min, 62°C 1 min, 72°C 2 min, 72°C 10 min | ||||
| Antisense: cagagctgcgcctgcagag | ||||||||
| TGF-β2 | Sense: ttcgcaggtatcgatggcac | 214 | 30 | 94°C 5 min, 94°C 1 min, 62°C 1 min 30, 72°C 2 min, 72°C 10 min | ||||
| Antisense: gcataattgctgccttcgcc | ||||||||
| TGF-β3 | Sense: ccagctccaagcgcacag | 164 | 28 | 94°C 5 min, 94°C 1 min, 64°C 1 min 30, 72°C 2 min, 72°C 10 min | ||||
| Antisense: gtgcgcgagtggctgttg | ||||||||
| Cyclin E | Sense: ctgaccattgtgtcctggct | 385 | 30 | 94°C 5 min, 94°C 1 min, 60°C 1 min 30, 72°C 2 min, 72°C 10 min | ||||
| Antisense: cagcttggacttgctggaca | ||||||||
| RPL19 | Sense: agtatgctcaggcttcagaa | 501 | 30 | 94°C 5 min, 94°C 1 min, 56°C 1 min 30, 72°C 2 min, 72°C 10 min | ||||
| Antisense:gcaggtctaagaccaaggaa | ||||||||