FACS analysis (%) | |
Wild-type | |
IEL | |
TCR-αβ | 49.65 (4.84) |
TCR-γδ | 51.26 (6.36) |
LPL | |
TCR-αβ | 65.72 (7.17) |
TCR-γδ | 18.05 (1.92) |
MIP-2 transgenic | |
IEL | |
TCR-αβ | 59.30 (3.41)1-150 |
TCR-γδ | 46.49 (4.36) |
LPL | |
TCR-αβ | 68.99 (5.54) |
TCR-γδ | 13.70 (2.27)1-150 |
Values are mean (SD).
FACScan analysis was performed on intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) with antimouse TCR-αβ and TCR-γδ antibodies, to determine the subpopulation of these cells. Single colour flow cytometric analysis was performed for FACScan. The percentage of positive cells was calculated in comparison with a negative control consisting of cells stained with FITC conjugated antirat IgG alone. Six mice each of wild-type mice and MIP-2 transgenic mice were examined.
↵1-150 p<0.05 versus wild-type mice.