Effect of LB-SCS treatment at a concentration which did not affect bacterial viability or adhesion on C1845 induced rearrangements of brush border associated structural and functional proteins in enterocyte-like Caco-2/TC7 cells
| Assay | Control uninfected | C1845 infected | C1845 infected LB-SCS treated |
|---|---|---|---|
| RI, relative immunofluorescence intensity expressed as arbitrary units, measured at the apical domain by conventional immunofluorescence microscopy in unpermeabilised cells. | |||
| Band, size of the immunofluorescent band (μm) measured by confocal laser scanning microscopy in permeabilised cells (vertical x– z optical section). | |||
| SI, AP, DPPIV, GLUT5, sucrase-isomaltase, alkaline phosphatase, dipeptidylpeptidase IV, and fructose transporter, respectively; LB-SCS, spent culture supernatant of Lactobacillusacidophilus LB culture. | |||
| Data are mean (SEM) from three experiments. | |||
| Statistical analysis was performed using a Student's t test: *not significant, **p<0.01 compared with control uninfected cells. | |||
| F-actin | |||
| RI | 2.78 (0.18) | 0.32 (0.09)** | 2.70 (0.20)* |
| SI | |||
| RI | 2.78 (0.32) | 0.40 (0.14)** | 2.71 (0.25)* |
| Band (μm) | 1.4–1.7 | 4.9–5.8 | 1.5–2.0 |
| AP | |||
| RI | 2.44 (0.21) | 1.21 (0.15)** | 2.39 (0.22)* |
| Band (μm) | 1.5–1.8 | 4.5–5.7 | 1.6–2.2 |
| DPP IV | |||
| RI | 2.84 (0.25) | 0.42 (0.21)** | 2.78 (0.28)* |
| Band (μm) | 1.8–2.0 | 4.3–4.7 | 1.8–2.1 |
| GLUT5 | |||
| RI | 2.30 (0.17) | 1.07 (0.15)** | 2.07 (0.25)* |
| Band (μm) | 1.6–2.0 | 5.3–6.7 | 1.7–2.2 |