Clinicopathological information on subjects providing tissue specimens and a summary of immunohistochemical findings
| Specimen category* | Age (y)/ sex | Histological grade of inflammation | Disease duration (y) | Lymphoid aggregates† | No of BCA-1+ CD21+ aggregates‡ | No of BCA-1+ CD21− aggregates | No of BCA-1+ DRC+ aggregates | No of BCA-1+ DRC− aggregates |
|---|---|---|---|---|---|---|---|---|
| *UC, ulcerative colitis; NC, normal colon. | ||||||||
| †Number of lymphoid aggregates per section judged by haematoxylin and eosin staining. Parallel sections from the same tissue block were evaluated (cut a distance of approximately 750 μm apart) and the numbers from the two sections are presented as a range. | ||||||||
| ‡Parallel sections 8 μm apart were examined for BCA-1+ follicles: one section was stained with polyclonal anti-BCA-1 in combination with anti-CD21; the other section was stained with monoclonal anti-BCA-1 in combination with the DRC antibody. Follicles were described as BCA-1+ when a reticular staining meshwork was seen; CD21+ or DRC+ aggregates were always surrounded by a BCA-1+ reticular meshwork. | ||||||||
| ¶Exstensive cell infiltration was seen but separate lymphoid aggregates were difficult to identify. | ||||||||
| BCA-1, B cell attracting chemokine 1. | ||||||||
| UC 1 | 28/F | 3 | 10 | Confluent¶ | 0 | 0 | 0 | 0 |
| UC 2 | 60/F | 3 | 14 | 7–8 | 1 | 1 | 1 | 0 |
| UC 3 | 32/M | 2 | 14 | 8–7 | 5 | 0 | 5 | 0 |
| UC 4 | 44/F | 2 | 2 | 3–4 | 4 | 0 | 4 | 0 |
| UC 5 | 12/M | 3 | 1 | 12–24 | 13 | 7 | 9 | 8 |
| NC 1 | 38/F | 0 | 0–1 | 1 | 0 | 0 | 1 | |
| NC 2 | 23/F | 0 | 0–3 | 3 | 0 | 3 | 0 | |
| NC 3 | 33/F | 0 | 0–3 | 2 | 0 | 2 | 0 | |