Characteristics of the immunoreagents used in the study
| Specificity | Clone | Immunoglobulin class/subclass | Origin of antibody | Conjugate/labelling | Producer/source |
|---|---|---|---|---|---|
| BCA-1, B cell attracting chemokine 1; CXCR5, CXC chemokine receptor 5; DRC, dendritic reticulum cell; vWF, Von Willebrandt factor; MAdCAM-1, mucosal addressin cell adhesion molecule 1; SMA, smooth muscle actin. | |||||
| BCA-1 | 53610.11 | IgG1 | Mouse | None | R&D Systems, Oxon, UK |
| BCA-1 | IgG | Goat | None | R&D Systems | |
| CXCR5 | 51505.111 | IgG2b | Mouse | None | R&D Systems |
| CD3 | Ig | Rabbit | None | Dako, Glostrup, Denmark | |
| CD20 | L26 | IgG2a | Mouse | None | Dako |
| CD21 | 1F8 | IgG1 | Mouse | None | Dako |
| CD23 | EBV CS-5 | IgG1 | Mouse | None | Becton-Dickinson, San Jose, CA, USA |
| CD35 | To5 | IgG1 | Mouse | None | Dako |
| MAdCAM-1 | 10G3 | IgG2a | Mouse | None | Courtesy of Michael Briskin |
| MAdCAM-1 | 10A6 | IgG1 | Mouse | None | Courtesy of Michael Briskin |
| DRC | R4/23 | IgM | Mouse | None | Dako |
| IgD | IgD26 | IgG1 | Mouse | None | Dako |
| vWF | Ig | Rabbit | None | Dako | |
| Muscle actin | HHF35 | IgG1 | Mouse | None | Dako |
| SMA | 1A4 | IgG2a | Mouse | None | Dako |
| Fibronectin | Ig | Rabbit | None | Dako | |
| Collagen IV | CIV 22 | IgG1 | Mouse | None | Dako |
| Mouse IgG1 | IgG | Goat | Cy3 (red) | Southern Biotechnology, Birmingham, AL, USA | |
| Mouse IgG2a | IgG | Goat | Biotinylated | Southern Biotechnology | |
| Mouse IgM | IgG | Goat | Biotinylated | Southern Biotechnology | |
| Goat IgG | Ig | Donkey | Alexa 546 (red) | Molecular Probes, Eugene, OR, USA | |
| Rabbit IgG | IgG | Goat | Alexa 488 (green) | Molecular Probes | |
| Mouse IgG1 | Ig | Rabbit | Alexa 488 | Zymed Laboratories, San Francisco, CA, USA. Molecular Probes | |
| Mouse IgG | IgG | Horse | Biotinylated | Vector Laboratories, Burkingame, CA, USA | |
| Mouse IgG | Ig | Donkey | Cy2 (green) | Jackson Immuno Research, West Grove, PA, USA | |
| Cy3 labelled streptavidin | Jackson | ||||
| Cy2 labelled streptavidin | Ammersham, Life Sciences, UK | ||||
| Alexa 350 labelled (blue) streptavidin | Molecular Probes | ||||