Induction of expression of various immune recognition molecules on biliary epithelial cells (BECs) by incubation with anti-BECs antibodies for 12 hours
| Antibodies to | Unstim BECs | TNF-α+IFN-γ stim BECs | Stim BECs+ IgM fraction from PSC sera | Stim BECs+ IgG fraction from PSC sera | Stim BECs+ IgM fraction from PBC sera | Stim BECs+ IgM fraction from AIH sera | Stim BECs+ IgM fraction from normal sera |
|---|---|---|---|---|---|---|---|
| +, >10–25; ++, >26–50; +++, >51–75 mean fluorescence channels compared with negative controls (only secondary antibodies). | |||||||
| PSC, primary sclerosing cholangitis; PBC, primary biliary cirrhosis; AIH, autoimmune hepatitis; TNF-α, tumour necrosis factor α; IFN-γ, interferon γ; stim, stimulated; unstim, unstimulated. | |||||||
| HLA -DR | − | +++ (only after 3 days (with IFN-γ) | − | − | − | − | − |
| CD80 | − | − | − | − | − | − | − |
| CD44 | + | + | +++ | +++/++++ | +/++ | + | + |
| CD58 | − | − | − | − | − | − | − |
| CD40 | − | − | − | − | − | − | − |
| ICAM-1 | − | ++ | − | − | − | − | − |