Table 1

Cell line controls for immunohistochemistry

	Positive controls			Negative controls
Gene	Cell line	Status	Reference	Cell line	Status	Reference
p16^INK4A	MDAMB 157	Overexpressed	35	MDAMB 231	Deleted	35
DPC4/Smad4	Hep G2	Wild-type	37	MDAMB 468	Deleted	23
p53	DU 145	Mutated	34	PC 3	Not expressed	34
p21^CIP1	HMEC 184	Moderate expression	36	BT 549	Very low expression	35
p27^KIP1	BT 474	Overexpressed	35	HMEC 184	Weak expression	36
Cyclin D1	MCF 7	High expresstion	35	HBL 100	Low expression	35
pRb	MCF 7	Wild-type	35	MDAMB 468	Mutated	35