Gene array analysis | In situ hybridisation* | |||||
---|---|---|---|---|---|---|
MMP | PWM (fold change v controls) | αCD3+IL-12 (fold change v controls) | Performed in this study | Control | PWM | αCD3+IL-12 |
The gene arrays detected MMP and TIMP genes in day 4 cultured explants, the fold change over control were quantified by computer. | ||||||
*In situ hybridisation signal was evaluated using dark field microscopy. | ||||||
Signal strength was assessed as follows: −, no detectable specific signal; +, specific signal in few cells; ++, specific signal in moderate number of cells; +++, specific signal in a high number of cells | ||||||
†In situ hybridisation data were shown in our previous study.17 | ||||||
ND, not determined; PWM, pokeweed mitogen; IL-12, interleukin-12. | ||||||
Collagenase 1 (MMP-1)† | 3.8 | 2.6 | Yes | − | +++ | + |
Gelatinase A (MMP-2) | −1.1 | 1.6 | No | ND | ND | ND |
Stromelysin 1 (MMP-3)† | 8.2 | 5.7 | Yes | + | +++ | ++ |
Matrilysin (MMP-7) | −1.9 | −2.0 | Yes | − | ++ | + |
Neutrophil collagenase (MMP-8) | −1.4 | 1.0 | No | ND | ND | ND |
Gelatinase B (MMP-9) | 6.2 | 5.5 | No | ND | ND | ND |
Stromelysin 2 (MMP-10) | 4.0 | 2.6 | Yes | − | +++ | ++ |
Stromelysin 3 (MMP-11) | −1.1 | 1.1 | No | ND | ND | ND |
Macrophage metalloelastase (MMP-12) | 11.9 | 7.3 | Yes | + | +++ | ++ |
Collagenase 3 (MMP13) | 1.2 | 1.5 | Yes | − | − | + |
MT1-MMP (MMP-14) | −1.0 | −1.2 | Yes | + | + | + |
MT2-MMP (MMP-15) | −1.4 | −1.0 | No | ND | ND | ND |
MT3-MMP (MMP-16) | −1.7 | −1.4 | No | ND | ND | ND |
MT4-MMP (MMP-17) | 1.7 | 2.7 | No | ND | ND | ND |
MMP-19 | −1.2 | 1.1 | No | ND | ND | ND |
TIMP-1 | −1.4 | −1.1 | Yes | +++ | + | + |
TIMP-2 | −1.0 | −1.1 | No | ND | ND | ND |
TIMP-3 | 1.5 | 1.1 | Yes | +++ | + | + |
TIMP-4 | 1.7 | 1.1 | No | ND | ND | ND |