Table 2

Sequence specific primers and their reaction conditions

Target sequencePrimersReaction conditions
APC gene5′-AAGCCTACCAATTATAGTGAACG-3′95°C for 15 min; 65 cycles (40 + 25) of 94°C for 1 min, 58°C for 1 min, 72°C for 1 min; and 2 min at 72°C
435 bp5′-AGCTGATGACAAAGATGATAATG-3′
APC gene5′-GGA CTACAGGCCATTGCAGAA-3′95°C for 15 min; 65 cycles (40 + 25) of 94°C for 1 min, 55°C for 1 min, 72°C for 1 min; and 2 min at 72°C
133 bp5′-GGCTACATCTCCAAAAGTCAA-3′
IS9005′-GAAGGGTGTTCGGGGCCGTCGCTTAGG-3′95°C for 15 min; 40 cycles of 94°C for 1 min, 58°C for 1 min, 72°C for 3 min; and 72°C for 2 min
413 bp outer5′-GGCGTTGAGGTCGATCGCCCACGTGAC-3′
IS9005′-CAGGGACGTCGGGTATGGCTTTCA-3′95°C for 15 min; 30 cycles of 94°C for 1 min, 55°C for 1 min, 72°C for 3 min; and 72°C for 2 min
328 bp nested5′-CGTCACCGCCGCAATCAACTCCAG-3′
IS9005′-GCCCGGATGCGCCACGACTT-3′95°C for 15 min; 40 cycles of 94°C for 1 min, 68°C for 1 min, 72°C for 30 sec; and 2 min at 72°C
193 bp outer5′-GCGCGGCACGGCTCTTGTTGTA-3′
IS9005′-GCGCCACGACTTGCAGCCTCTG-3′95°C for 15 min; 30 cycles of 94°C for 1 min, 68°C for 30 sec, 72°C for 30 sec; and 2 min at 72°C
155 bp nested5′-CGCGTTCCAGCGCCGAAAGTATT-3′