APC gene | 5′-AAGCCTACCAATTATAGTGAACG-3′ | 95°C for 15 min; 65 cycles (40 + 25) of 94°C for 1 min, 58°C for 1 min, 72°C for 1 min; and 2 min at 72°C |
435 bp | 5′-AGCTGATGACAAAGATGATAATG-3′ | |
APC gene | 5′-GGA CTACAGGCCATTGCAGAA-3′ | 95°C for 15 min; 65 cycles (40 + 25) of 94°C for 1 min, 55°C for 1 min, 72°C for 1 min; and 2 min at 72°C |
133 bp | 5′-GGCTACATCTCCAAAAGTCAA-3′ | |
IS900 | 5′-GAAGGGTGTTCGGGGCCGTCGCTTAGG-3′ | 95°C for 15 min; 40 cycles of 94°C for 1 min, 58°C for 1 min, 72°C for 3 min; and 72°C for 2 min |
413 bp outer | 5′-GGCGTTGAGGTCGATCGCCCACGTGAC-3′ | |
IS900 | 5′-CAGGGACGTCGGGTATGGCTTTCA-3′ | 95°C for 15 min; 30 cycles of 94°C for 1 min, 55°C for 1 min, 72°C for 3 min; and 72°C for 2 min |
328 bp nested | 5′-CGTCACCGCCGCAATCAACTCCAG-3′ | |
IS900 | 5′-GCCCGGATGCGCCACGACTT-3′ | 95°C for 15 min; 40 cycles of 94°C for 1 min, 68°C for 1 min, 72°C for 30 sec; and 2 min at 72°C |
193 bp outer | 5′-GCGCGGCACGGCTCTTGTTGTA-3′ | |
IS900 | 5′-GCGCCACGACTTGCAGCCTCTG-3′ | 95°C for 15 min; 30 cycles of 94°C for 1 min, 68°C for 30 sec, 72°C for 30 sec; and 2 min at 72°C |
155 bp nested | 5′-CGCGTTCCAGCGCCGAAAGTATT-3′ | |