Concentrations (mean (SEM)) of glucose and lactate and pH in media of 105 or 106 transformed colonic epithelial cells after 24 hours of culture
| 105 cells | 106 cells | |||||
|---|---|---|---|---|---|---|
| Glucose (mM) | Lactate (mM) | pH | Glucose (mM) | Lactate (mM) | pH | |
| HT-29 cells were cultured for 24 hours in 2.5 ml of Dulbecco’s modified Eagle’s medium with 10% fetal calf serum containing glucose 5.5 mM (with or without butyrate 5 mM) or 25 mM. During culture, the media were left unchanged or changed every eight hours, as indicated. | ||||||
| n=5 for all experiments. | ||||||
| *p<0.05 compared with cells cultured in media containing glucose 5.5 mM without butyrate and changed every eight hours (paired t test). | ||||||
| †p<0.05 compared with cells cultured at identical conditions without butyrate (paired t test). | ||||||
| Initial concentration of glucose | ||||||
| Media unchanged | ||||||
| 5.5 mM | 5.0 (0.1) | 2.1 (0.2) | 7.46 (0.02) | 1.8 (0.1)* | 8.0 (0.1)* | 7.33 (0.01)* |
| 5.5 mM+butyrate | 5.1 (0.1) | 1.7 (0.1) | 7.48 (0.02) | 2.8 (0.3) † | 6.2 (0.4) † | 7.31 (0.02) |
| 25 mM | 21 (0.4) | 2.3 (0.2) | 7.44 (0.02) | 17 (0.4)* | 7.4 (0.1)* | 7.23 (0.01)* |
| Media changed every 8 hours | ||||||
| 5.5 mM | 5.3 (0.1) | 1.9 (0.2) | 7.45 (0.02) | 4.3 (0.1) | 2.5 (0.2) | 7.41 (0.02) |
| 5.5 mM+butyrate | 5.2 (0.1) | 1.4 (0.1) | 7.45 (0.03) | 3.6 (0.2) † | 4.2 (0.2) † | 7.32 (0.01) † |