Primers used for mutation screening of CARD4 coding sequence and sizes of the corresponding polymerase chain reaction products
| Exon | Forward primer | Backward primer | Size (bp) |
|---|---|---|---|
| The large exon 3 was screened by amplification of six overlapping DNA fragments. | |||
| 1 | TCATTGATCTCTTCAGGGGC | GGTCCACACAATGCCATGC | 378 |
| 2 | CTTATGGTAGGAGGATGCCC | ACTGGTGCCTGCGGTCTTG | 271 |
| 3 | TGATGCTGTTTCTGCCTCTC | AATTTGACCCCTGCGTCTAG | 374 |
| 3 | TGAGACCATCTTCATCCTGG | CTTCCCACTGAGCAGGTTG | 379 |
| 3 | GTGCCTGACAGCTCCTGC | CAGGGTCATCGTGCAGTCG | 372 |
| 3 | CTCTTCTGCTGGATCATCTTC | GTGGAAAAACTCATAGGACTG | 376 |
| 3 | GGCTTCCTGCGGGCTTTG | GGAAAACAGGTGTGCCCAC | 392 |
| 3 | CAGCCCTGAGGAGAAAGCG | CCACCTGTTGCTCCCCTTG | 403 |
| 4 | CATAATGAGTGCCTGCCCTG | GCCATCCGTGCCGATCATC | 235 |
| 5 | ACATCAGGAGCCAGAAAGTC | CAGAAGGGGGTGATCAAGAG | 183 |
| 6 | AGGAGGGTGACCCACATTG | CCACACACACAGCAGGTTG | 199 |
| 7 | CTTGGGGAGCTAACATTCAC | GAGCACGAATCACCCTTCC | 208 |
| 8 | GCTAAGATTGAATGTAGATGTG | CAAACAAACAAATGAAATGACTC | 175 |
| 9 | CGCTCCTGTGAACTCTAAAAC | AAAGTAGCACAGTCTGAAATTG | 197 |
| 10 | TCAAATTTGATCCAGTATTATGG | GTGGTGGTGAGTAAACAGTC | 193 |
| 11 | ACCTGTGTACTTTTGTCTTCC | TTTGCTGCTGAGGCTCCAG | 158 |