List of primer sets used for evaluation of the Helicobacter genus infection, MSP, and RT-PCR
| Name | Sense primer | Antisense primer |
|---|---|---|
| RT-PCR, reverse transcriptase-polymerase chain reaction; MSP, methylation specific PCR. | ||
| H pylori | ||
| 1st | 5′-AGGATGAAGGTTTTAGGATT-3′ | 5′-CTGGAGAGACTAAGCCCTCC-3′ |
| 2nd | 5′-ATTACTGACGCTGATTGTGC-3′ | 5′-CTGGAGAGACTAAGCCCTCC-3′ |
| Helicobacter genus | ||
| 1st | 5′-CGTGGAGGATGAAGGTTTTA-3′ | 5′-TACACCAAGAATTCCACCTA-3′ |
| 2nd | 5′-CGTGGAGGATGAAGGTTTTA-3′ | 5′-AATTCCACCTACCTCTCCC-3′ |
| MSP | ||
| p15-M | 5′-GCGTTCGTATTTTGCGGTT-3′ | 5′-CGTACAATAACCGAACGACCGA-3′ |
| p15-U | 5′-TGTGATGTGTTTGTATTTTGTGGTT-3′ | 5′-CCATACAATAACCAAACAACCAA-3′ |
| p16-M | 5′-TTATTAGAGGGTGGGGCGGATCGC-3′ | 5′-GACCCCGAACCGCGACCGTAA-3′ |
| p16-U | 5′-TTATTAGAGGGTGGGGTGGATTGT-3′ | 5′-CAACCCCAAACCACAACCATAA-3′ |
| p73-M | 5′-GGACGTAGCGAAATCGGGGTTC-3′ | 5′-ACCCCGAACATCGACGTCCG-3′ |
| p73-U | 5′-AGGGGATGTAGTGAAATTGGGGTTT-3′ | 5′-ATCACAACCCCAAACATCAACATCCA-3′ |
| hMLH1-M | 5′-TTAATAGGAAGAGCGGATAGC-3′ | 5′-CTATAAATTACTAAATCTCTTCG-3′ |
| hMLH1-U | 5′-TTAATAGGAAGAGTGGATAGTG-3′ | 5′-TCTATAAATTACTAAATCTCTTCA-3′ |
| MINT1-M | 5′-AATTTTTTTATATATATTTTCGAAGC-3′ | 5′-AAAAACCTCAACCCCGCG-3′ |
| MINT1-U | 5′-AATTTTTTTATATATATTTTTGAAGTGT-3′ | 5′-AACAAAAAACCTCAACCCCACA-3′ |
| MINT2-M | 5′-TTGTTAAAGTGTTGAGTTCGTC-3′ | 5′-AATAACGACGATTCCGTACG-3′ |
| MINT2-U | 5′-GATTTTGTTAAAGTGTTGAGTTTGTT-3′ | 5′-CAAAATAATAACAACAATTCCATACA-3′ |
| MINT31-M | 5′-TGTTGGGGAAGTGTTTTTCGGC-3′ | 5′-CGAAAACGAAACGCCGCG-3′ |
| MINT31-U | 5′-TAGATGTTGGGGAAGTGTTTTTTGGT-3′ | 5′-TAAATACCCAAAAACAAAACACCACA-3′ |
| DAP kinase-M | 5′-GGATAGTCGGATCGAGTTAACGTC-3′ | 5′-CCCTCCCAAACGCCGA-3′ |
| DAP kinase-U | 5′-GGAGGATAGTTGGATTGAGTTAATGTT-3′ | 5′-CAAATCCCTCCCAAACACCAA-3′ |
| RT-PCR | ||
| t(11;18)(q21;q21) | 5′-TTCATCCGTCAAGTTCAAGC-3′ | 5′-TTGAACAAAAGGATGTCCAG-3′ |
| 1st | 5′-TTACTCAATGCAGAAGATGA-3′ | 5′-ACTGTAAAACCAATGTGCTG-3′ |
| 5′-CAAGAGGAACTGATTGATACG-3′ | 5′-TTCCTATCAAAAGGGCAACC-3′ | |
| t(11;18)(q21;q21) | 5′-AGCCAGTTACCCTCATCTAC-3′ | 5′-CAGCCAAGACTGCCTTTGAC-3′ |
| 2nd-A | 5′-GGCATCAGCTTTTGGGAAGT-3′ | |
| 5′-AAAGGCTGGTCAGTTGTTTG-3′ | ||
| t(11;18)(q21;q21) | 5′-GAAATAAGGGAAGAGGAGAG-3′ | 5′-CAGCCAAGACTGCCTTTGAC-3′ |
| 2nd-B | 5′-GGCATCAGCTTTTGGGAAGT-3′ | |
| 5′-AAAGGCTGGTCAGTTGTTTG-3′ | ||
| t(11;18)(q21;q21) | 5′-ATTGCAGCCACTGTATTCAG-3′ | 5′-CAGCCAAGACTGCCTTTGAC-3′ |
| 2nd-C | 5′-GGCATCAGCTTTTGGGAAGT-3′ | |
| 5′-AAAGGCTGGTCAGTTGTTTG-3′ | ||